دورية أكاديمية
Longitudinal analysis of invariant natural killer T cell activation reveals a cMAF-associated transcriptional state of NKT10 cells
| العنوان: | Longitudinal analysis of invariant natural killer T cell activation reveals a cMAF-associated transcriptional state of NKT10 cells |
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| المؤلفون: | Harry Kane, Nelson M LaMarche, Áine Ní Scannail, Amanda E Garza, Hui-Fern Koay, Adiba I Azad, Britta Kunkemoeller, Brenneth Stevens, Michael B Brenner, Lydia Lynch |
| المصدر: | eLife, Vol 11 (2022) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | iNKT cells, activation, transcriptional remodeling, adipose tissue, scRNA-Seq, cellular metabolism, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Innate T cells, including CD1d-restricted invariant natural killer T (iNKT) cells, are characterized by their rapid activation in response to non-peptide antigens, such as lipids. While the transcriptional profiles of naive, effector, and memory adaptive T cells have been well studied, less is known about the transcriptional regulation of different iNKT cell activation states. Here, using single-cell RNA-sequencing, we performed longitudinal profiling of activated murine iNKT cells, generating a transcriptomic atlas of iNKT cell activation states. We found that transcriptional signatures of activation are highly conserved among heterogeneous iNKT cell populations, including NKT1, NKT2, and NKT17 subsets, and human iNKT cells. Strikingly, we found that regulatory iNKT cells, such as adipose iNKT cells, undergo blunted activation and display constitutive enrichment of memory-like cMAF+ and KLRG1+ populations. Moreover, we identify a conserved cMAF-associated transcriptional network among NKT10 cells, providing novel insights into the biology of regulatory and antigen-experienced iNKT cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| العلاقة: | https://elifesciences.org/articles/76586Test; https://doaj.org/toc/2050-084XTest |
| DOI: | 10.7554/eLife.76586 |
| الوصول الحر: | https://doaj.org/article/13c9d127f6404c0f9e370cc5ce5f8c3bTest |
| رقم الانضمام: | edsdoj.13c9d127f6404c0f9e370cc5ce5f8c3b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.76586 |