دورية أكاديمية
Calcium Signal-Initiated Early Activation of NF-kappa B in Neurons Is a Neuroprotective Event in Response to Kainic Acid-Induced Excitotoxicity
| العنوان: | Calcium Signal-Initiated Early Activation of NF-kappa B in Neurons Is a Neuroprotective Event in Response to Kainic Acid-Induced Excitotoxicity |
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| المؤلفون: | Li, Shu-Yan, Sun, Wen-Ge, Jia, Yu-Hong, Wu, Guo-Sheng, An, Guo-Shun, Ni, Ju-Hua, Jia, Hong-Ti |
| المساهمون: | Li, SY (reprint author), Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Xue Yuan Rd 38, Beijing 100191, Peoples R China., Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China., Chifeng Coll, Sch Med, Dept Biochem, Chi Feng 024001, Peoples R China., Capital Univ Med Sci, Dept Biochem & Mol Biol, Beijing 100054, Peoples R China., Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Xue Yuan Rd 38, Beijing 100191, Peoples R China. |
| المصدر: | EI ; SCI |
| بيانات النشر: | biochemistry moscow |
| سنة النشر: | 2010 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | NF-kappa B, kainic acid, ROS, calcium, AMPA/KA receptor, neuronal survival/death, NUCLEAR EXPORT SIGNAL, TRANSCRIPTION FACTOR, NEURODEGENERATIVE DISORDERS, HIPPOCAMPAL-NEURONS, CEREBRAL-ISCHEMIA, OXIDATIVE STRESS, STRIATAL NEURONS, GENE-EXPRESSION, PROTEIN-KINASE, CELL-DEATH |
| الوصف: | We demonstrate that activation of nuclear factor kappa B (NF-kappa B) in neurons is neuroprotective in response to kainic acid (KA)-induced excitotoxicity. Combination of Western blotting, immunocytochemistry, and electrophoresis mobility shift assay showed that KA exposure induced a fast but transient nuclear translocation of the NF-kappa B p65 subunit and increased DNA-binding activity of NF-kappa B in primary cultured cortical neurons. The transient NF-kappa B activity was associated with upregulation of antiapoptotic Bcl-xL and XIAP gene products revealed by real-time PCR. Knockdown of p65 decreased neuronal viability and antiapoptotic gene expression. In addition, we showed that KA-stimulated DNA-binding activity of NF-kappa B was associated with reactive oxygen species and calcium signals, using AMPA/KA receptor antagonist, calcium chelator, and antioxidant. These results suggest that the fast and transient activation of NF-kappa B initiated by calcium signals is one of the important proximal events in response to KA-induced excitotoxicity, which has neuroprotective effect against KA-induced apoptosis. ; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000274738400013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Test ; Biochemistry & Molecular Biology ; SCI(E) ; EI ; 3 ; 1 ; 101-U1 ; 75 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 0006-2979 |
| العلاقة: | BIOCHEMISTRY-MOSCOW.2010,75,(1),101-U1.; 936780; http://hdl.handle.net/20.500.11897/245614Test; WOS:000274738400013 |
| DOI: | 10.1134/S000629791001013X |
| الإتاحة: | https://doi.org/20.500.11897/245614Test https://doi.org/10.1134/S000629791001013XTest https://hdl.handle.net/20.500.11897/245614Test |
| رقم الانضمام: | edsbas.E28D7CEE |
| قاعدة البيانات: | BASE |
| تدمد: | 00062979 |
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| DOI: | 10.1134/S000629791001013X |