التفاصيل البيبلوغرافية
| العنوان: |
Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungs. |
| المؤلفون: |
Lemieszek, Marta Kinga, Rzeski, Wojciech, Golec, Marcin, Mackiewicz, Barbara, Zwoliński, Jacek, Dutkiewicz, Jacek, Milanowski, Janusz |
| المصدر: |
Ecotoxicology & Environmental Safety; May2020, Vol. 194, pN.PAG-N.PAG, 1p |
| مصطلحات موضوعية: |
MYOFIBROBLASTS, HYPERSENSITIVITY pneumonitis, PULMONARY fibrosis, LUNGS, CONNECTIVE tissues, EPITHELIAL cells, MICE |
| مصطلحات جغرافية: |
POLAND |
| مستخلص: |
Pantoea agglomerans is gram-negative bacteria widely distributed in nature. It predominates in inhalable dust from grain, herbs, and flax, and was identified as the most important cause of hypersensitivity pneumonitis (HP) in eastern Poland. To better understand the molecular mechanism of HP development studies focused on the interactions between P. agglomerans and alveolar epithelial cells as well as lung tissue with particular emphasis on the epithelial-mesenchymal transition (EMT). The studies were conducted on human normal lung epithelial NL20 cells and mice strain C57BL/6J. Cells and mice underwent chronic exposure to saline extract of P. agglomerans (SE-PA). Morphological changes were evaluated under light microscopy, the concentration of fibrosis markers was examined by the ELISA method, while the expression of genes involved in EMT was evaluated by RealTime PCR. During incubation with SE-PA epithelial cells underwent conversion and assumed fibroblast phenotype characterized by a decrease in epithelial cells markers (CDH1 , CLDN1 , JUP) and increase in mesenchymal cells markers (FN1 , VIM , CDH2). Mice lungs collected after 14 days of SE-PA treatment revealed inflammation with marked lymphocytes infiltration. The intensified inflammatory process accompanied by increased proliferation of fibrous connective tissue was noted in mice lungs after 28 days of SE-PA exposure. Histological changes correlated with an increase of fibrosis markers (hydroxyproline, collagens), downregulation of epithelial markers (Cdh1 , Cldn1 , Jup , Ocln) and upregulation of myofibroblasts markers (Acta2 , Cdh2, Fn1 , Vim). Obtained results revealed SE-PA ability to induce EMT in human lung epithelial cells and mice lung tissue, with the scale of changes proportional to the time of treatment. • Pantoea agglomerans induces epithelial-mesenchymal transition in vitro and in vivo. • Epithelial-mesenchymal transition induced by P. agglomerans is responsible for lung fibrosis. • Epithelial cells exposure to P. agglomerans as in vitro model of hypersensitivity pneumonitis. [ABSTRACT FROM AUTHOR] |
|
Copyright of Ecotoxicology & Environmental Safety is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Supplemental Index |
