From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats
| العنوان: | From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats |
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| المؤلفون: | Qing Wang, Wen-juan Miao, Rui Li, Xue Qiao, Cheng Xiang, Min Ye, Dean Guo |
| المصدر: | Drug Metabolism and Disposition. 39:1597-1608 |
| بيانات النشر: | American Society for Pharmacology & Experimental Therapeutics (ASPET), 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Male, Pharmacology, Spectrometry, Mass, Electrospray Ionization, Chromatography, Plant Extracts, Chemistry, Hydrolysis, Metabolite, Electrospray ionization, Pharmaceutical Science, Decoction, Crude drug, Tandem mass spectrometry, Mass spectrometry, Rats, Rats, Sprague-Dawley, chemistry.chemical_compound, Metabolic pathway, In vivo, Glycyrrhiza, Animals, Chromatography, High Pressure Liquid |
| الوصف: | Because of the complicated chemical composition of traditional Chinese medicines (TCMs), their metabolic study has been a great challenge, especially when they are used in the traditional way, i.e., clinical oral dose of water decoction. Poor understanding of metabolic pathways and too low metabolite concentrations to be detected in biosamples are the major hurdles. In the present work, a three-step strategy was proposed to systematically characterize in vivo metabolites of TCMs at a normal clinical dosage. Licorice, one of the most popular TCMs, was studied as a model. First, 10 representative compounds of licorice were administered to rats separately. A total of 68 metabolites were characterized by high-performance liquid chromatography coupled with diode-array detection and electrospray ionization tandem mass spectrometry and liquid chromatography (LC)/quadrupole time-of-flight-mass spectrometry (MS) analyses, together with enzyme hydrolysis. Among these, 13 compounds were confirmed by comparison with reference standards, including the 10 administered licorice compounds. Second, a high dose (equivalent to 20-fold clinical dosage) of licorice water extract was administered, and 22 more metabolites were characterized. Finally, these metabolites (including constituents of licorice) were determined by a highly sensitive and selective LC/selected reaction monitoring-MS method when the licorice water decoction was orally administered to rats at a clinical dosage (0.9 g crude drug/kg). A total of 42 metabolites in plasma and 62 metabolites in urine were detected. This is the first attempt to fully profile the in vivo metabolites of licorice at a normal clinical dosage. |
| تدمد: | 1521-009X 0090-9556 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c248f629ef2d278bf399ea79973d9a2Test https://doi.org/10.1124/dmd.111.038695Test |
| رقم الانضمام: | edsair.doi.dedup.....6c248f629ef2d278bf399ea79973d9a2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1521009X 00909556 |
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