XPA deficiency affects the ubiquitin-proteasome system function
| العنوان: | XPA deficiency affects the ubiquitin-proteasome system function |
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| المؤلفون: | Vandeclecio Lira da Silva, Tirzah Braz Petta Lajus, Lucymara Fassarella Agnez-Lima, Sandro J. de Souza, Riva de Paula Oliveira, Ana Rafaela de Souza Timoteo, Cesar Orlando Muñoz-Cadavid, Lázaro Batista de Azevedo Medeiros, Julliane Tamara Araújo de Melo Campos, Angélica Maria de Sousa Leal, Ana Helena Sales de Oliveira, Andre Luis Fonseca Faustino |
| المصدر: | Repositório Institucional da UFRN Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | endocrine system, Proteasome Endopeptidase Complex, Xeroderma pigmentosum, DNA Repair, NF-E2-Related Factor 2, Down-Regulation, Biology, medicine.disease_cause, Biochemistry, 03 medical and health sciences, Transcriptional regulation, 0302 clinical medicine, RNA interference, Gene expression, medicine, DNA-(Apurinic or Apyrimidinic Site) Lyase, Humans, Molecular Biology, Cells, Cultured, 030304 developmental biology, 0303 health sciences, Proteasome, Ubiquitin, Gene Expression Profiling, Cell Biology, Apurinic-apyrimidinic endonuclease 1 (APE1), medicine.disease, NFE2L2, Cell biology, Xeroderma Pigmentosum Group A Protein, Oxidative Stress, Proteostasis, Gene Expression Regulation, Oxidative stress, 030220 oncology & carcinogenesis, Proteolysis |
| الوصف: | Xeroderma pigmentosum complementation group A (XPA), is defective in xeroderma pigmentosum patients, causing pre-disposition to skin cancer and neurological abnormalities, which is not well understood. Here, we analyzed the XPA-deficient cells transcriptional profile under oxidative stress. The imbalance in of ubiquitin-proteasome system (UPS) gene expression was observed in XPA-deficient cells and the involvement of nuclear factor erythroid 2-related factor-2 (NFE2L2) was indicated. Co-immunoprecipitation assays showed the interaction between XPA, apurinic-apyrimidinic endonuclease 1 (APE1) and NFE2L2 proteins. Decreased NFE2L2 protein expression and proteasome activity was also observed in XPA-deficient cells. The data suggest the involvement of the growth arrest and DNA-damage-inducible beta (GADD45β) in NFE2L2 functions. Similar results were obtained in xpa-1 (RNAi) Caenorhabditis elegans suggesting the conservation of XPA and NFE2L2 interactions. In conclusion, stress response activation occurs in XPA-deficient cells under oxidative stress; however, these cells fail to activate the UPS cytoprotective response, which may contribute to XPA patient’s phenotypes. |
| تدمد: | 1568-7856 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e851c4be264d84c92a501315002e598Test https://pubmed.ncbi.nlm.nih.gov/32693352Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7e851c4be264d84c92a501315002e598 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15687856 |
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