AMPK Activation Inhibits Expression of Proinflammatory Mediators Through Downregulation of PI3K/p38 MAPK and NF-κB Signaling in Murine Macrophages
| العنوان: | AMPK Activation Inhibits Expression of Proinflammatory Mediators Through Downregulation of PI3K/p38 MAPK and NF-κB Signaling in Murine Macrophages |
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| المؤلفون: | Chun-Hsiang Lin, Jiun-Tsai Lin, Yi-Fang Cheng, Bee-Piao Huang, Shao-Hsuan Kao, Han-Min Chen |
| المصدر: | DNA and Cell Biology. 34:133-141 |
| بيانات النشر: | Mary Ann Liebert Inc, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Lipopolysaccharides, Adenosine monophosphate, Cell Survival, Immunoblotting, Down-Regulation, Gene Expression, AMP-Activated Protein Kinases, p38 Mitogen-Activated Protein Kinases, Cell Line, Nitric oxide, Proinflammatory cytokine, Mice, chemistry.chemical_compound, Genetics, Animals, Phosphorylation, Protein kinase A, Molecular Biology, biology, Plant Extracts, Reverse Transcriptase Polymerase Chain Reaction, Macrophages, NF-kappa B, AMPK, Interleukin, Cell Biology, General Medicine, Cell biology, Enzyme Activation, Plant Leaves, Nitric oxide synthase, chemistry, Immunology, biology.protein, Cytokines, lipids (amino acids, peptides, and proteins), Tumor necrosis factor alpha, Inflammation Mediators, Phosphatidylinositol 3-Kinase, Sasa, Signal Transduction |
| الوصف: | Adenosine monophosphate (AMP)-activated protein kinase (AMPK) plays a central role in energy homeostasis and regulation of inflammatory responses. The present study is aimed to investigate the anti-inflammatory effects of ENERGI-F704, a nucleobase analogue isolated from bamboo leaves, on expression of proinflammatory mediators in murine macrophage RAW264.7 in response to lipopolysaccharide (LPS). ENERGI-F704 enhanced phosphorylation of AMPK(T172) but insignificantly affected the viability of RAW264.7 cells. Further investigation showed that ENERGI-F704 decreased mRNA expression of interleukin (IL)-6, IL-8, tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX2), and inducible nitric oxide synthase (iNOS) induced by LPS, as well as suppressed the production of prostaglandin E2 (PGE₂) and nitric oxide (NO). Additionally, the inhibitory effects of ENERGI-F704 on the LPS-induced proinflammatory mediators were diminished by pretreatment of AMPK inhibitor Compound C. ENERGI-F704 also inhibited LPS-triggered activation of nuclear factor kappa B (NF-κB), phosphatidylinositol 3-kinase (PI3K), and p38 mitogen-activated protein kinase (p38), whereas extracellular signal-regulated kinase (Erk)1/2 and c-Jun N-terminal kinase (JNK) were insignificantly influenced. Our findings indicate that ENERGI-F704 may exert anti-inflammatory activity on RAW264.7 cells in response to LPS through the activation of AMPK and suppression of PI3K/P38/NF-κB signaling and the consequent decreased expression of proinflammatory mediators, suggesting that ENERGI-F704 is beneficial to the amelioration of inflammatory disorders. |
| تدمد: | 1557-7430 1044-5498 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4180501fb10c9d1a64b78e70a90a3cfTest https://doi.org/10.1089/dna.2014.2630Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....b4180501fb10c9d1a64b78e70a90a3cf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15577430 10445498 |
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