Performance of a Novel Blood-Based Early Colorectal Cancer Screening Assay in Remaining Serum after the Blood Biochemical Test
| العنوان: | Performance of a Novel Blood-Based Early Colorectal Cancer Screening Assay in Remaining Serum after the Blood Biochemical Test |
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| المؤلفون: | Ying Chen, Yong Ma, Hongchun Li, Linyan Zhang, Guodong Zhao, Chuang Sun, Zhenzhen Wang, Minxue Zheng |
| المصدر: | Disease Markers Disease Markers, Vol 2019 (2019) |
| بيانات النشر: | Hindawi, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, medicine.medical_specialty, Article Subject, Colorectal cancer, Clinical Biochemistry, Gastroenterology, Polymerase Chain Reaction, Sensitivity and Specificity, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Genetics, Biomarkers, Tumor, Medicine, Humans, Molecular Biology, Aged, lcsh:R5-920, Crc screening, business.industry, Biochemistry (medical), Cancer, General Medicine, Methylation, DNA Methylation, Middle Aged, medicine.disease, Serum samples, 030104 developmental biology, Molecular Diagnostic Techniques, Colorectal cancer screening, 030220 oncology & carcinogenesis, DNA methylation, Female, Syndecan-2, business, lcsh:Medicine (General), Colorectal Neoplasms, Septins, Research Article |
| الوصف: | Background. Combination of multiple biomarkers was an effective strategy to improve sensitivity in cancer diagnosis and screening. However, the performance of the combination of methylated SEPT9 and SDC2 for detection of colorectal cancer (CRC) has yet to be reported. Methods. A new qPCR-based assay combining the detection of methylated SEPT9 and SDC2 was used. Methylation statuses of SEPT9 and SDC2 were examined in 19 sets of cancer tissues and paired adjacent tissues and further evaluated with 225 serum samples, including 111 CRC patients and 114 no evidence of disease individuals. Results. SEPT9 and SDC2 methylation levels were higher in 94.7% and 100.0% of cancer tissues than in their paired adjacent tissues. The sensitivities for detecting CRC by SEPT9 methylation alone and SDC2 methylation alone were 73.0% (95% CI: 63.6–80.8%) and 71.2% (95% CI: 61.8–79.2%), respectively, with the same specificity of 95.6% (95% CI: 89.6–98.4%). However, when SEPT9 methylation was combined with SDC2 methylation to detect CRC, the sensitivity was improved to 86.5% (95% CI: 78.4–92.0%) with a specificity of 92.1% (95% CI: 85.1–96.1%). Conclusion. The combination of methylated SEPT9 and SDC2 detection in serum has the potential to be a noninvasive strategy for CRC screening. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 1875-8630 0278-0240 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::052f3199e859a120c44b79b0d1c9badcTest http://europepmc.org/articles/PMC6476029Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....052f3199e859a120c44b79b0d1c9badc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18758630 02780240 |
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