Mapping the Segmental Microbiomes in the Human Small Bowel in Comparison with Stool: A REIMAGINE Study
| العنوان: | Mapping the Segmental Microbiomes in the Human Small Bowel in Comparison with Stool: A REIMAGINE Study |
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| المؤلفون: | Laith H. Jamil, Gillian M. Barlow, Rashin Sedighi, Ali Rezaie, Gabriela Leite, Ruchi Mathur, Maritza Sanchez, Simon K. Lo, Shreya Celly, Stacy Weitsman, Walter Morales, Gonzalo Parodi, Shirley C Paski, Mark Pimentel, Maria Jesus Villanueva-Millan |
| المصدر: | Digestive Diseases and Sciences |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Male, Adolescent, Duodenum, Physiology, Firmicutes, Ribotyping, Microbiology, Feces, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Intestine, Small, medicine, Humans, 16S metagenomic analysis, Prospective Studies, Microbiome, Aged, Aged, 80 and over, Bacteria, biology, Lachnospiraceae, Gastroenterology, Bacteroidetes, Middle Aged, biology.organism_classification, Small intestine, Gastrointestinal Microbiome, Jejunum, medicine.anatomical_structure, Stool, 030220 oncology & carcinogenesis, Female, Original Article, 030211 gastroenterology & hepatology, Neisseriaceae, Metagenomics, Proteobacteria, Ruminococcaceae |
| الوصف: | Background Most gut microbiome studies have been performed using stool samples. However, the small intestine is of central importance to digestion, nutrient absorption, and immune function, and characterizing its microbial populations is essential for elucidating their roles in human health and disease. Aims To characterize the microbial populations of different small intestinal segments and contrast these to the stool microbiome. Methods Male and female subjects undergoing esophagogastroduodenoscopy without colon preparation were prospectively recruited. Luminal aspirates were obtained from the duodenum, jejunum, and farthest distance reached. A subset also provided stool samples. 16S rRNA sequencing was performed and analyses were carried out using CLC Genomics Workbench. Results 16S rRNA sequencing identified differences in more than 2000 operational taxonomic units between the small intestinal and stool microbiomes. Firmicutes and Proteobacteria were the most abundant phyla in the small intestine, and Bacteroidetes were less abundant. In the small intestine, phylum Firmicutes was primarily represented by lactic acid bacteria, including families Streptococcaceae, Lactobacillaceae, and Carnobacteriaceae, and Proteobacteria was represented by families Neisseriaceae, Pasteurellaceae, and Enterobacteriaceae. The duodenal and FD microbial signatures were markedly different from each other, but there were overlaps between duodenal and jejunal and between jejunal and FD microbial signatures. In stool, Firmicutes were represented by families Ruminococcaceae, Lachnospiraceae, Christensenellaceae, and Proteobacteria by class Deltaproteobacteria. Conclusions The small bowel microbiome is markedly different from that in stool and also varies between segments. These findings may be important in determining how compositional changes in small intestinal microbiota contribute to human disease states. Electronic supplementary material The online version of this article (10.1007/s10620-020-06173-x) contains supplementary material, which is available to authorized users. |
| تدمد: | 1573-2568 0163-2116 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13a224254c1fc8fc5b8eb7b8df17c633Test https://doi.org/10.1007/s10620-020-06173-xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....13a224254c1fc8fc5b8eb7b8df17c633 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15732568 01632116 |
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