Cyclosporin A Reduces the Severity of Cold-Restraint-Induced Gastric Lesions: Role of Leukocytes
| العنوان: | Cyclosporin A Reduces the Severity of Cold-Restraint-Induced Gastric Lesions: Role of Leukocytes |
|---|---|
| المؤلفون: | Tangül Şan, İnci Alican, Berrak Ç. Yeğen, Hizir Kurtel, Şule Çetinel, Tamer Coskun |
| المصدر: | Digestion. 56:214-219 |
| بيانات النشر: | S. Karger AG, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Male, Restraint, Physical, medicine.medical_specialty, Neutrophils, Premedication, Ischemia, Granulocyte, Thiobarbituric Acid Reactive Substances, Rats, Sprague-Dawley, Pathogenesis, Lipid peroxidation, chemistry.chemical_compound, Stress, Physiological, Cyclosporin a, Internal medicine, medicine, TBARS, Animals, Stomach Ulcer, Peroxidase, biology, business.industry, Immune Sera, Gastroenterology, medicine.disease, Rats, Cold Temperature, Endocrinology, medicine.anatomical_structure, chemistry, Gastric Mucosa, Myeloperoxidase, Immunology, Cyclosporine, Microscopy, Electron, Scanning, biology.protein, Female, business, Infiltration (medical) |
| الوصف: | The objective of this study was to determine the role of cyclosporin A (CsA) on cold-restraint-induced gastric lesions. Animals were subjected to 3 h immobilization at 4 degrees C in plastic restraining devices following a starvation period of 48 h. Gastric samples were obtained for the measurement of myeloperoxidase (MPO) activity, an index of number of peroxidase positive cells and thiobarbituric acid-reactive substances (TBARS; lipid peroxidation). Animals were pretreated with CsA which is a potent immunosuppressant and inhibits ischemia/reperfusion-induced polymorphonuclear leukocyte (PMN) infiltration. Cold-restraint administration significantly elevated the tissue MPO activity and TBARS formation. CsA pretreatment significantly reduced the severity of cold-restraint-induced gastric lesions while attenuating the elevated MPO measurements observed during cold-restraint administration. Animals rendered neutropenic with antineutrophil serum (ANS) exhibited significantly less gastric mucosal injury normally observed after cold-restraint stress. Neither CsA nor ANS treatment effected the elevated TBAR levels, indicating that PMNs are not involved in the lipid peroxidation process observed after cold-restraint stress. In conclusion, the results of this study indicate that CsA is capable of inhibiting cold-restraint-induced gastric mucosal injury and can attenuate the cold-restraint-induced increases in gastric MPO measurements. Our results also indicate that PMNs may be the important mediators of cold-restraint-induced gastric lesions. |
| تدمد: | 1421-9867 0012-2823 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f907d8af1674491dbb5389851b19e950Test https://doi.org/10.1159/000201246Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....f907d8af1674491dbb5389851b19e950 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14219867 00122823 |
|---|