Plasmacytoid Precursor Dendritic Cells From NOD Mice Exhibit Impaired Function
| العنوان: | Plasmacytoid Precursor Dendritic Cells From NOD Mice Exhibit Impaired Function |
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| المؤلفون: | Larry D. Bozulic, Yiming Huang, Paula M. Chilton, Mary Jane Elliott, Suzanne T. Ildstad, Hong Xu, Thomas Miller, Isabelle Fugier-Vivier |
| المصدر: | Diabetes |
| بيانات النشر: | American Diabetes Association, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Male, CD8 Antigens, Endocrinology, Diabetes and Metabolism, Antigens, CD19, Receptors, Antigen, T-Cell, Nod, CD19, Immunophenotyping, Mice, 03 medical and health sciences, 0302 clinical medicine, Adjuvants, Immunologic, Mice, Inbred NOD, Precursor cell, Internal Medicine, medicine, Animals, Antigens, Ly, Lectins, C-Type, 030304 developmental biology, NOD mice, 0303 health sciences, CD11b Antigen, biology, T-cell receptor, Hematopoietic Stem Cell Transplantation, Membrane Proteins, Hematopoietic stem cell, hemic and immune systems, Dendritic Cells, Dendritic cell, Flow Cytometry, Hematopoietic Stem Cells, Up-Regulation, Cell biology, Diabetes Mellitus, Type 1, medicine.anatomical_structure, Antigens, Surface, Immunology, biology.protein, Cytokines, Female, lipids (amino acids, peptides, and proteins), Immunology and Transplantation, Biomarkers, CD8, NK Cell Lectin-Like Receptor Subfamily B, 030215 immunology |
| الوصف: | OBJECTIVE—Plasmacytoid precursor dendritic cell facilitating cells (p-preDC FCs) play a critical role in facilitation of syngeneic and allogeneic hematopoietic stem cell (HSC) engraftment. Here, we evaluated the phenotype and function of CD8+/TCR− FCs from NOD mice.RESEARCH DESIGN AND METHODS—The phenotype of CD8+/TCR− FCs was analyzed by flow cytometry using sorted FCs from NOD, NOR, or B6 mice. The function of NOD FCs was evaluated by colony-forming cell (CFC) assay in vitro and syngeneic or allogeneic HSC transplantation in vivo.RESULTS—We report for the first time that NOD FCs are functionally impaired. They fail to facilitate engraftment of syngeneic and allogeneic HSCs in vivo and do not enhance HSC clonogenicity in vitro. NOD FCs contain subpopulations similar to those previously described in B6 FCs, including p-preDC, CD19+, NK1.1+DX5+, and myeloid cells. However, the CD19+ and NK1.1+DX5+ subpopulations are significantly decreased in number in NOD FCs compared with disease-resistant controls. Removal of the CD19+ or NK1.1+DX5+ subpopulations from FCs did not significantly affect facilitation. Notably, Flt3 ligand (FL) treatment of NOD donors expanded FC total in peripheral blood and restored facilitating function in vivo.CONCLUSIONS—These data demonstrate that NOD FCs exhibit significantly impaired function that is reversible, since FL restored production of functional FCs in NOD mice and suggest that FL plays an important role in the regulation and development of FC function. FCs may therefore be linked to diabetes pathogenesis and prevention. |
| تدمد: | 1939-327X 0012-1797 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd41e0db9edd2357f1af3118a949dbe9Test https://doi.org/10.2337/db08-0356Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dd41e0db9edd2357f1af3118a949dbe9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1939327X 00121797 |
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