التفاصيل البيبلوغرافية
| العنوان: |
Chemokine receptor CCR3 is important for migration of mast cells in neurofibroma. |
| المؤلفون: |
Tai, Wei-Chun, Wang, Sin-Ting, Wu, Chieh-Shan, Lin, Tze-Yi, Wu, Meng-Tse |
| المصدر: |
Dermatologica Sinica; Dec2010, Vol. 28 Issue 4, p146-153, 8p |
| مصطلحات موضوعية: |
CHEMOKINES, CELL migration, EXTRACELLULAR matrix, MAST cells, FIBROBLASTS, NEUROFIBROMA, ENZYME-linked immunosorbent assay |
| مستخلص: |
Abstract: Background: Neurofibroma consists of abundant extracellular matrix and many types of cells, including Schwann cells (SCs), mast cells (MCs), fibroblasts and endothelial cells. As SCs have been found to be the cell of origin for neurofibroma, how MCs may migrate into the tumor has not been fully clarified. Given that chemokine receptor CCR3 is found predominantly expressed by differentiated MCs, we postulated that CCR3 may play a role in the homing of MCs to neurofibroma. The goal of this study is to investigate the possible involvement of chemokine receptor CCR3 in the migration of MCs to the neurofibroma. Methods: Expressional and functional assays for CCR3 and its ligands were performed on MCs and SCs. Results: By real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, we found one of the CCR3 ligand, CCL7 was highly expressed by murine SC cell line SW10, and also moderately expressed by MCs. In serial chemotaxis assays, MCs were found specifically responsive to CCL7 and also condition medium from SW10 cells, indicating SCs may attract MCs by CCR3-mediated cell migration. Conclusion: The interaction of CCR3 and CCL7 may play important roles for MC migration toward SC in the neurofibroma. [ABSTRACT FROM AUTHOR] |
|
Copyright of Dermatologica Sinica is the property of Wolters Kluwer India Pvt Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Supplemental Index |
Full Text Finder