دورية أكاديمية
Correlation of hepatitis C RNA and serum alanine aminotransferase in hepatitis B and C seronegative healthy blood donors
| العنوان: | Correlation of hepatitis C RNA and serum alanine aminotransferase in hepatitis B and C seronegative healthy blood donors |
|---|---|
| المؤلفون: | Ali, Natasha Bahadur, Moiz, Bushra, Moatter, Tariq, Ahmed, Shiraz, Adil, Salman, Khurshid, Mohammad |
| المصدر: | Department of Pathology and Laboratory Medicine |
| بيانات النشر: | eCommons@AKU |
| سنة النشر: | 2010 |
| المجموعة: | The Aga Khan University: eCommons@AKU |
| مصطلحات موضوعية: | Hepatitis C, Serum alanine aminotransferase, HCV RNA, Hematology, Microbiology, Pathology |
| الوصف: | Introduction: Historically, serum alanine transaminase (ALT) has been used as a surrogate marker in the detection of hepatitis viruses in blood donors. With the availability of newer sensitive technologies for the detection of seroconversion, the value of ALT becomes questionable but continues to be used for this purpose with subsequent discarding of ALT elevated blood units.Objective: The present study aims to evaluate the significance and cost effectiveness of ALT as a surrogate marker for hepatitis C virus infection in healthy asymptomatic blood donors who were serologically negative.Materials and methods: The study was conducted at clinical laboratory of a tertiary care hospital for a period of one year from November 2006 to October 2007. All donors were screened serologically for hepatitis B, C and HIV I and II, syphilis and malaria and those tested positive were excluded from further evaluation. Gender-wise reference ranges and minimal and markedly raised results for ALT (described respectively as one and two folds increase above reference range) were defined and, accordingly, donors were grouped into three. Two hundred seronegative blood donors were randomly selected from all three groups of ALT results and tested for hepatitis C nucleic acid through Amplicor HCV RNA test. The cost of discarding an ALT -only elevated blood unit was also assessed. During the study period, 25117 subjects donated blood. EIGHT HUNDRED AND RESULTS: Seventy two donors (3.4%) were positive for one or more serological tests. ALT of all donors ranged from 0-1501 U/L (Mean +/- SD; 33.4 +/- 25.45 U/L). The donors seronegative for all disease markers were 24245 (96.6%). Of these, 21164 (87.2%) donors had their ALT within reference range while 2874 (11.8%) and 207 (0.8%) of donors had minimal and markedly elevated results. Thus, 621 blood bags (red cells, platelets and plasma) costing $ 39200.0 were discarded based on ALT results alone. Of 200 seronegative donors evaluated for hepatitis C nucleic acid, only one within markedly ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://ecommons.aku.edu/pakistan_fhs_mc_pathol_microbiol/83Test; http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2010;volume=53;issue=3;spage=480;epage=485;aulast=AliTest |
| الإتاحة: | https://ecommons.aku.edu/pakistan_fhs_mc_pathol_microbiol/83Test http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2010;volume=53;issue=3;spage=480;epage=485;aulast=AliTest |
| رقم الانضمام: | edsbas.33C355BA |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |