المؤلفون: Linda R Wang Lam, Stephen Romas, Jack G. Caton, Xinping Zhang, Ravi S. Misra, Zhuang Zhou, Kevin Schilling

المصدر: Dent Mater J

مصطلحات موضوعية: Materials science, Periodontal ligament stem cells, Periodontal Ligament, 0206 medical engineering, Nanofibers, ALIZARIN RED, 02 engineering and technology, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Osteogenesis, Enamel matrix derivative, Bovine serum albumin, Bone regeneration, General Dentistry, Cell Proliferation, biology, Cell Differentiation, 030206 dentistry, 020601 biomedical engineering, Membrane, chemistry, Nanofiber, Polycaprolactone, Guided Tissue Regeneration, Periodontal, Ceramics and Composites, biology.protein, Biomedical engineering

الوصف: The osteogenic effect of a composite electrospun core-shell nanofiber membrane encapsulated with Emdogain® (EMD) was evaluated. The membrane was developed through coaxial electrospinning using polycaprolactone as the shell and polyethylene glycol as the core. The effects of the membrane on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) were examined using Alizarin Red S staining and qRT-PCR. Characterization of the nanofiber membrane demonstrated core-shell morphology with a mean diameter of ~1 µm. Examination of the release of fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) from core-shell nanofibers over a 22-day period showed improved release profile of encapsulated proteins as compared to solid nanofibers. When cultured on EMD-containing core-shell nanofibers, PDLSCs showed significantly improved osteogenic differentiation with increased Alizarin Red S staining and enhanced osteogenic gene expression, namely OCN, RUNX2, ALP, and OPN. Core-shell nanofiber membranes may improve outcomes in periodontal regenerative therapy through simultaneous mechanical barrier and controlled drug delivery function.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b32cbef1e98b85895ff916ad76cecfc3Test
https://doi.org/10.4012/dmj.2020-412Test

المؤلفون: Pierre Weiss, Anne-Claire Marsden, Xavier Struillou, Mia Rakic, S. Portron, Christian Verner, Assem Soueidan, Zahi Badran

المساهمون: Jehan, Frederic, Regenerative Medicine and Skeleton (RMeS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Département de parodontologie [UNIV Nantes] (Faculté de chirurgie dentaire), Université de Nantes (UN)

المصدر: Dental Materials Journal
Dental Materials Journal, Japanese Society for Dental Materials and Devices, 2019, Epub ahead of print. ⟨10.4012/dmj.2018-315⟩

مصطلحات موضوعية: Scaffold, Future studies, Materials science, Periodontal Ligament, 0206 medical engineering, Biocompatible Materials, 02 engineering and technology, Bioinformatics, Regenerative Medicine, Regenerative medicine, 03 medical and health sciences, 0302 clinical medicine, medicine, Periodontal fiber, Animals, Humans, Cementum, General Dentistry, Dental Cementum, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Periodontal regeneration, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Regeneration (biology), Mesenchymal stem cell, Mesenchymal Stem Cells, 030206 dentistry, Biomaterial, 020601 biomedical engineering, medicine.anatomical_structure, Systematic review, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Ceramics and Composites, Pre-clinical study

الوصف: International audience; The aim of the systematic review was to analyze the use of mesenchymal stem cells (MSC) and biomaterial for periodontal regeneration from preclinical animal models and human. Electronic databases were searched and additional hand-search in leading journals was performed. The research strategy was achieved according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The including criteria were as follows: MSC, biomaterial, in vivo studies, with histologic and radiologic analysis and written in English. The risk of bias was assessed for individual studies. A total of 50 articles were selected and investigated in the systematic review. These results indicate that MSC and scaffold provide beneficial effects on periodontal regeneration, with no adverse effects of such interventions. Future studies need to identify the suitable association of MSC and biomaterial and to characterize the type of new cementum and the organization of the periodontal ligament fiber regeneration.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f0251360f8f1719756b4a1ef102935eTest
https://pubmed.ncbi.nlm.nih.gov/31511473Test

المؤلفون: Febe Carolina Vázquez-Vázquez, Marco Antonio Alvarez-Perez, Amaury Pozos-Guillén, José Luis Suárez-Franco, Octavio Alvarez-Fregoso, Juan José Montesinos

المصدر: Dental materials journal. 37(3)

مصطلحات موضوعية: Adult, Scaffold, Materials science, Biocompatibility, Adolescent, Cell Survival, Periodontal Ligament, Surface Properties, Polyesters, Nanofibers, Biocompatible Materials, 02 engineering and technology, Microscopy, Atomic Force, 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, Tissue engineering, Materials Testing, Cell Adhesion, Periodontal fiber, Humans, Bicuspid, Fiber, General Dentistry, Cell Proliferation, Tissue Engineering, Tissue Scaffolds, technology, industry, and agriculture, Mesenchymal Stem Cells, 030206 dentistry, Adhesion, respiratory system, equipment and supplies, 021001 nanoscience & nanotechnology, Polyester, Nanofiber, Ceramics and Composites, Microscopy, Electron, Scanning, lipids (amino acids, peptides, and proteins), 0210 nano-technology, Biomedical engineering

الوصف: This study evaluated the influence in the biocompatibility of human periodontal ligament (hPDL) mesenchymal stromal cell onto poly lactic-acid (PLA) films and PLA fiber membrane. Fiber scaffold was prepared via air jet spinning (AJS) from PLA solutions (6, 7, and 10%) and analyzed using SEM, AFM and FTIR. Biocompatibility was evaluated by adhesion, proliferation and cell-material interaction. PLA film exhibited a smooth and homogenously surface topography in comparison with random orientation of PLA fiber with roughness structure where diameter size depends on PLA solution. Moreover, cell adhesion; proliferation and cell-material interaction has the best respond on random orientation nanofiber of 10, followed by 7, and 6% of PLA in comparison with PLA films. It could be concluded that AJS is an attractive alternative technique for manufacture fiber scaffolds with a tunable random orientation geometry of fibers that allow to produce interconnected porous formed by nanometric fiber diameter structures that could be a potential scaffold for periodontal tissue engineering applications.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77ff13bfebc0b3ede33ba620fbceef8bTest
https://pubmed.ncbi.nlm.nih.gov/29553121Test

المؤلفون: Libor Borák, Natsuko Murakami, Zdenek Florian, Patrik Prachár, Masahiro Ona, Noriyuki Wakabayashi, Yoshimasa Igarashi, Sonia Bartáková

المصدر: Dental materials journal. 30(4)

مصطلحات موضوعية: Materials science, Periodontal Ligament, Movement, 0206 medical engineering, Finite Element Analysis, Dentistry, Temporal Muscle, 02 engineering and technology, Mandible, Models, Biological, Bite Force, Stress (mechanics), 03 medical and health sciences, 0302 clinical medicine, Imaging, Three-Dimensional, stomatognathic system, Tooth Apex, Elastic Modulus, Premolar, medicine, Alveolar Process, Image Processing, Computer-Assisted, Periodontal fiber, Humans, Odontometry, Bicuspid, Computer Simulation, Tooth Root, Tooth Socket, General Dentistry, Elastic modulus, Orthodontics, Tooth Crown, business.industry, Masseter Muscle, Pterygoid Muscles, 030206 dentistry, 020601 biomedical engineering, Finite element method, Biomechanical Phenomena, stomatognathic diseases, Skull, medicine.anatomical_structure, Ceramics and Composites, Cortical bone, Stress, Mechanical, business

الوصف: This study aimed to introduce a procedure for determining the bilinear elastic moduli (E1 and E2) of the periodontal ligament for a mathematical tooth model to analyse stress in the mandible. The bone and tooth morphology were scanned from a dry skull and an extracted intact tooth, respectively, and reconstructed in a three-dimensional finite element model. The model showed good agreement with previously reported in vivo premolar movement when an E1 for the first phase tooth movement of 0.05 MPa and an E2 for the second phase of 8.0 MPa with ε(12) of 0.075 were adopted. The mandible model analysis indicated that a remarkably high maximum compressive stress in the cervical cortical bone and the tensile stress in areas of masticatory muscle attachment were found. Future stress analyses using a jaw model may follow the process of determination of bilinear moduli to enhance accurate simulation with less calculation time.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e8d829ff9472c29d8fa3ce8690f5ddaTest
https://pubmed.ncbi.nlm.nih.gov/21778610Test