Electrospun core-shell nanofibers with encapsulated enamel matrix derivative for guided periodontal tissue regeneration
| العنوان: | Electrospun core-shell nanofibers with encapsulated enamel matrix derivative for guided periodontal tissue regeneration |
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| المؤلفون: | Linda R Wang Lam, Stephen Romas, Jack G. Caton, Xinping Zhang, Ravi S. Misra, Zhuang Zhou, Kevin Schilling |
| المصدر: | Dent Mater J |
| بيانات النشر: | Japanese Society for Dental Materials and Devices, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Materials science, Periodontal ligament stem cells, Periodontal Ligament, 0206 medical engineering, Nanofibers, ALIZARIN RED, 02 engineering and technology, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Osteogenesis, Enamel matrix derivative, Bovine serum albumin, Bone regeneration, General Dentistry, Cell Proliferation, biology, Cell Differentiation, 030206 dentistry, 020601 biomedical engineering, Membrane, chemistry, Nanofiber, Polycaprolactone, Guided Tissue Regeneration, Periodontal, Ceramics and Composites, biology.protein, Biomedical engineering |
| الوصف: | The osteogenic effect of a composite electrospun core-shell nanofiber membrane encapsulated with Emdogain® (EMD) was evaluated. The membrane was developed through coaxial electrospinning using polycaprolactone as the shell and polyethylene glycol as the core. The effects of the membrane on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) were examined using Alizarin Red S staining and qRT-PCR. Characterization of the nanofiber membrane demonstrated core-shell morphology with a mean diameter of ~1 µm. Examination of the release of fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) from core-shell nanofibers over a 22-day period showed improved release profile of encapsulated proteins as compared to solid nanofibers. When cultured on EMD-containing core-shell nanofibers, PDLSCs showed significantly improved osteogenic differentiation with increased Alizarin Red S staining and enhanced osteogenic gene expression, namely OCN, RUNX2, ALP, and OPN. Core-shell nanofiber membranes may improve outcomes in periodontal regenerative therapy through simultaneous mechanical barrier and controlled drug delivery function. |
| تدمد: | 1881-1361 0287-4547 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b32cbef1e98b85895ff916ad76cecfc3Test https://doi.org/10.4012/dmj.2020-412Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b32cbef1e98b85895ff916ad76cecfc3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18811361 02874547 |
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