Development of a Rapid Focus Reduction Neutralization Test Assay for Measuring SARS-CoV-2 Neutralizing Antibodies
| العنوان: | Development of a Rapid Focus Reduction Neutralization Test Assay for Measuring SARS-CoV-2 Neutralizing Antibodies |
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| المؤلفون: | Pei Yong Shi, Robert C. Kauffman, Grace Mantus, Jens Wrammert, Mehul S. Suthar, Evan J. Anderson, Venkata Viswanadh Edara, Vineet D. Menachery, Abigail Vanderheiden, Nadine Rouphael, Katharine Floyd, Sri Edupuganti |
| المصدر: | Curr Protoc Immunol Current Protocols in Immunology |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), viruses, Immunology, Neutralization, Virus, Article, 03 medical and health sciences, 0302 clinical medicine, Immunity, Neutralization Tests, Neutralization test, Pandemic, Chlorocebus aethiops, Medicine, Animals, Humans, skin and connective tissue diseases, Pandemics, Vero Cells, biology, business.industry, SARS-CoV-2, fungi, virus diseases, COVID-19, General Medicine, Vaccine efficacy, Virology, Antibodies, Neutralizing, body regions, 030104 developmental biology, biology.protein, Antibody, business, 030215 immunology |
| الوصف: | SARS-CoV-2 is a recently emerged human coronavirus that has escalated to a pandemic. There are currently no approved vaccines for SARS-CoV-2, which causes severe respiratory illness or death. Defining the antibody response to SARS-CoV-2 will be essential for understanding disease progression, long-term immunity, and vaccine efficacy. Here we describe two methods for evaluating the neutralization capacity of SARS-CoV-2 antibodies. The basic protocol is a focus reduction neutralization test (FRNT), which involves immunostaining infected cells with a chromogen deposit readout. The alternate protocol is a modification of the FRNT that uses an infectious clone-derived SARS-CoV-2 virus expressing a fluorescent reporter. These protocols are adapted for use in a high-throughput setting, and are compatible with large-scale vaccine studies or clinical testing. © 2020 Wiley Periodicals LLC Basic Protocol: Focus reduction neutralization test Alternate Protocol: mNeonGreen-based focus reduction neutralization test (FRNT-mNG). |
| تدمد: | 1934-368X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::326d9f111dfe0c3d7c16ff2958a73dceTest https://pubmed.ncbi.nlm.nih.gov/33215858Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....326d9f111dfe0c3d7c16ff2958a73dce |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1934368X |
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