The CCAAT/enhancer-binding protein–ATF response elements-luciferase mouse model, an innovative tool to monitor the integrated stress response pathway in vivo
| العنوان: | The CCAAT/enhancer-binding protein–ATF response elements-luciferase mouse model, an innovative tool to monitor the integrated stress response pathway in vivo |
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| المؤلفون: | Alain Bruhat, Pierre Fafournoux |
| المساهمون: | Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Centre de Recherche en Nutrition Humaine Rhône Alpes (CRNH Rhône Alpes), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA) |
| المصدر: | Current Opinion in Clinical Nutrition and Metabolic Care Current Opinion in Clinical Nutrition and Metabolic Care, 2017, 20 (3), pp.175-180. ⟨10.1097/MCO.0000000000000359⟩ Current Opinion in Clinical Nutrition and Metabolic Care, Lippincott, Williams & Wilkins, 2017, 20 (3), pp.175-180. ⟨10.1097/MCO.0000000000000359⟩ |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, mice, Cellular adaptation, Activating transcription factor, Medicine (miscellaneous), Protein Serine-Threonine Kinases, souris, Biology, 03 medical and health sciences, Stress, Physiological, réponse au stress, Animals, facteur de transcription ATF4, Integrated stress response, Luciferase, Phosphorylation, Luciferases, Muscle, Skeletal, Promoter Regions, Genetic, Transcription factor, Nutrition and Dietetics, Ccaat-enhancer-binding proteins, ATF4, muscle squelettique, Promoter, Activating Transcription Factor 4, Molecular biology, Cell biology, Muscular Atrophy, voluntary muscle, 030104 developmental biology, Models, Animal, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Signal Transduction |
| الوصف: | Purpose of review The article highlights the recent development of an ATF4 (activating transcription factor) inducible luciferase (LUC) mouse model to monitor the integrated stress response pathway (ISR) in vivo. Recent finding The ISR pathway plays a key role in cellular adaptation to stress and is dysregulated in numerous diseases. The core event in this pathway is the phosphorylation of eukaryotic translation initiation factor 2 α, which leads to the recruitment of the transcription factor ATF4 to specific CCAAT/enhancer-binding protein-ATF response elements (CAREs) located in the promoters of target genes. To monitor the modulation of this pathway in the whole animal and at tissue and cellular levels, we generated a CARE-driven LUC mouse model. We validated the relevance of this model to study stress-related pathologies and recently observed the correlation between the ISR pathway induction in muscle and the occurrence of stress-induced skeletal muscle atrophy. Summary The CARE-LUC mouse model represents an innovative tool for investigating the role of the ISR pathway in physiology and disease and opens new avenues for the development of drugs that could modify this important pathway in stress-related human diseases. |
| تدمد: | 1363-1950 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d0dceca63567871ed0747f052d32b35Test https://doi.org/10.1097/mco.0000000000000359Test |
| رقم الانضمام: | edsair.doi.dedup.....5d0dceca63567871ed0747f052d32b35 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13631950 |
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