دورية أكاديمية
Plasma Cabozantinib Level Measurement in Patients with Renal Cell and Hepatocellular Carcinomas Using a Simple HPLC–UV Method Suitable for Clinical Application
| العنوان: | Plasma Cabozantinib Level Measurement in Patients with Renal Cell and Hepatocellular Carcinomas Using a Simple HPLC–UV Method Suitable for Clinical Application |
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| المؤلفون: | Takeo Yasu, Yoshito Gando, Ryosuke Nishijima, Risa Ikuta, Motofumi Suzuki, Mikio Shirota |
| المصدر: | Current Oncology, Vol 30, Iss 5, Pp 4871-4879 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | cabozantinib, high-performance liquid chromatography–ultraviolet, plasma concentration, therapeutic drug monitoring, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Cabozantinib, which is used to treat renal cell and hepatocellular carcinomas, is often associated with dose-dependent adverse events. Monitoring the levels of cabozantinib in the blood may maximize the therapeutic effect and prevent serious adverse events. In this study, we developed a high-performance liquid chromatography–ultraviolet (HPLC–UV) method of measuring plasma cabozantinib concentration. Human plasma samples (50 µL) were processed by simple deproteinization with acetonitrile, followed by chromatographic separation on a reversed-phase column with an isocratic mobile phase of 0.5% KH₂PO4 (pH 4.5) and acetonitrile (43:57, v/v) at a flow rate of 1.0 mL/min, with a 250 nm ultraviolet detector. The calibration curve was linear over the concentration range (0.05–5 µg/mL) with a coefficient of determination of 0.99999. The accuracy of the assay ranged from −4.35% to 0.98%, and recovery was >96.04%. The measurement time was 9 min. These findings confirm the effectiveness of this HPLC–UV method for cabozantinib quantification in human plasma, which is sufficiently simple for use for monitoring patients in clinical settings. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 30050367 1718-7729 1198-0052 |
| العلاقة: | https://www.mdpi.com/1718-7729/30/5/367Test; https://doaj.org/toc/1198-0052Test; https://doaj.org/toc/1718-7729Test |
| DOI: | 10.3390/curroncol30050367 |
| الوصول الحر: | https://doaj.org/article/0327723aaf41435f97c5eed0094c0a1bTest |
| رقم الانضمام: | edsdoj.0327723aaf41435f97c5eed0094c0a1b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 30050367 17187729 11980052 |
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| DOI: | 10.3390/curroncol30050367 |