دورية أكاديمية
Primary Vitreoretinal Lymphoma: Current Diagnostic Laboratory Tests and New Emerging Molecular Tools
العنوان: | Primary Vitreoretinal Lymphoma: Current Diagnostic Laboratory Tests and New Emerging Molecular Tools |
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المؤلفون: | Beatrice Melli, Pietro Gentile, Davide Nicoli, Enrico Farnetti, Stefania Croci, Fabrizio Gozzi, Elena Bolletta, Luca De Simone, Francesca Sanguedolce, Andrea Palicelli, Maurizio Zizzo, Stefano Ricci, Fiorella Ilariucci, Cristiana Rossi, Alberto Cavazza, Stefano Ascani, Luca Cimino, Magda Zanelli |
المصدر: | Current Oncology, Vol 29, Iss 10, Pp 6908-6921 (2022) |
بيانات النشر: | MDPI AG, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
مصطلحات موضوعية: | lymphoma, vitreoretinal, IL-10, IGH, MYD88, NGS, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
الوصف: | Primary vitreoretinal lymphoma (PVRL), a rare aggressive malignancy primarily involving the retina and/or the vitreous, is a major diagnostic challenge for clinicians (who commonly misdiagnose it as chronic uveitis) as well as for pathologists (for biological and technical reasons). Delays in diagnosis and treatment are responsible for visual impairments and life-threatening consequences, usually related to central nervous system involvement. The identification of lymphoma cells in vitreous fluid, obtained by vitrectomy, is required for diagnosis. Of note, the scarcity of neoplastic cells in small volumes of vitreous sample, and the fragility of lymphoma cells with degenerative changes caused by previous steroid use for presumed uveitis makes diagnosis based on cytology plus immunophenotyping difficult. Interleukin levels, immunoglobulin heavy chain or T-cell receptor gene rearrangements, and MYD88 mutation are applied in combination with cytology to support diagnosis. We aim to describe the current laboratory technologies for PVRL diagnosis, focusing on the main issues that these methods have. In addition, new emerging diagnostic strategies, such as next-generation sequencing analysis, are discussed. The genetic profile of PVRL remains largely unexplored. Better knowledge of genetic alterations is critical for precision medicine interventions with target-based treatments of this lymphoma for which no standardised treatment protocol currently exists. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1718-7729 1198-0052 |
العلاقة: | https://www.mdpi.com/1718-7729/29/10/543Test; https://doaj.org/toc/1198-0052Test; https://doaj.org/toc/1718-7729Test |
DOI: | 10.3390/curroncol29100543 |
الوصول الحر: | https://doaj.org/article/d67278b362e5462aa0f23309b547f580Test |
رقم الانضمام: | edsdoj.67278b362e5462aa0f23309b547f580 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 17187729 11980052 |
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DOI: | 10.3390/curroncol29100543 |