Purification of grapevine flavescence dorée MLO (Mycoplasma-like organism) by immunoaffinity
| العنوان: | Purification of grapevine flavescence dorée MLO (Mycoplasma-like organism) by immunoaffinity |
|---|---|
| المؤلفون: | Antoine Caudwell, Abdessamad Seddas, Xavier Daire, Elisabeth Boudon-Padieu, Raymonde Meignoz |
| المصدر: | Current Microbiology. 27:229-236 |
| بيانات النشر: | Springer Science and Business Media LLC, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | Antigenicity, medicine.drug_class, General Medicine, Biology, Monoclonal antibody, Applied Microbiology and Biotechnology, Microbiology, Molecular biology, Epitope, chemistry.chemical_compound, Antigen, chemistry, Affinity chromatography, Polyclonal antibodies, medicine, biology.protein, Flavescence dorée, DNA |
| الوصف: | To date MLO (Mycoplasma-like organism) remain non-culturable organisms and are difficult to extract in good conditions of purity and conservation from infected hosts (plants or leafhopper vectors). An immunoaffinity procedure that permits the purification of large quantities of Grapevine Flavescence doree MLO (FD-MLO) is described, with covalently bound and oriented IgG molecules of a previously obtained anti-FD-MLO monoclonal antibody and elution of antigens in alkaline conditions. Evidence for purity and integrity of the eluted MLO is presented. The two main antigenic components detected by rabbit polyclonal antibodies to FD-MLO were shown to be different proteins and to contain different epitopes with the use of different monoclonal antibodies. DNA extracted from the purified FD-MLO fraction hybridized with an FD-MLO DNA-specific probe. |
| تدمد: | 1432-0991 0343-8651 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::a1726aaa2da4d1c75a6da5fb3f25c33dTest https://doi.org/10.1007/bf01692881Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........a1726aaa2da4d1c75a6da5fb3f25c33d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320991 03438651 |
|---|