Label-Free Quantitative Proteomic Reveals Differentially Expressed Proteins in Aeromonas-Immunostimulated Leukocytes of Lampetra japonica
| العنوان: | Label-Free Quantitative Proteomic Reveals Differentially Expressed Proteins in Aeromonas-Immunostimulated Leukocytes of Lampetra japonica |
|---|---|
| المؤلفون: | Tiesong Li, Yue Pang, Yu Zuo, Wenying Zhang, Qingwei Li, Ting Zhu, Yingying Li |
| المصدر: | Current Microbiology. 75:934-941 |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Fish Proteins, Proteomics, 0301 basic medicine, Proteome, Quantitative proteomics, Applied Microbiology and Biotechnology, Microbiology, Mass Spectrometry, law.invention, 03 medical and health sciences, Immune system, law, Leukocytes, Animals, Chromatography, High Pressure Liquid, Polymerase chain reaction, biology, Lamprey, Lampreys, General Medicine, biology.organism_classification, Acquired immune system, 030104 developmental biology, Biochemistry, Aeromonas, human activities |
| الوصف: | Lamprey was considered to be one of the most basal jawless vertebrate representatives for studying vertebrate evolution, embryo development, and the origin of adaptive immunity. Here we investigated the effect of the gut-derived Aeromonas on the lamprey leukocytes proteome using the label-free liquid chromatography-tandem mass spectrometry for quantitative proteomics analysis. Significant difference was observed in the regulation of 34 out of 755 proteins in Aeromonas-immunized lamprey. 31 proteins were only identified in saline solution-immunized lamprey and 47 proteins were only identified in Aeromonas-immunized lamprey. Quantitative real-time polymerase chain reaction was used to validate the results of the proteomic analysis. The differentially expressed proteins were found to be associated with several different biological processes. The identification of leukocytes proteins essential for lamprey adaptive immune response induced by gut-derived Aeromonas strain could supply important information on lamprey-Aeromonas interactions and VLR-based adaptive immune signal pathways. |
| تدمد: | 1432-0991 0343-8651 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04f4e8f43084b00ce9402146c35a623dTest https://doi.org/10.1007/s00284-018-1468-2Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....04f4e8f43084b00ce9402146c35a623d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320991 03438651 |
|---|