NIT2, the nitrogen regulatory protein of Neurospora crassa, binds upstream of nia, the tomato nitrate reductase gene, in vitro
| العنوان: | NIT2, the nitrogen regulatory protein of Neurospora crassa, binds upstream of nia, the tomato nitrate reductase gene, in vitro |
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| المؤلفون: | Hoai-Nam Truong, Gabor Jarai, George A. Marzluf, Françoise Daniel-Vedele |
| المساهمون: | Laboratoire de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| المصدر: | Current Genetics Current Genetics, Springer Verlag, 1992, 21, pp.37-41 |
| سنة النشر: | 1992 |
| مصطلحات موضوعية: | 0106 biological sciences, Recombinant Fusion Proteins, Mutant, [SDV.GEN] Life Sciences [q-bio]/Genetics, Nitrate reductase, GENE NIA, 01 natural sciences, Neurospora, Nitrate Reductase, Neurospora crassa, Fungal Proteins, 03 medical and health sciences, Nitrate Reductases, Genetics, Promoter Regions, Genetic, Gene, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Regulation of gene expression, 0303 health sciences, [SDV.GEN]Life Sciences [q-bio]/Genetics, biology, fungi, Structural gene, food and beverages, Promoter, Zinc Fingers, General Medicine, DNA, Plants, biology.organism_classification, DNA-Binding Proteins, Biochemistry, 010606 plant biology & botany, Transcription Factors |
| الوصف: | The nit-2 gene of Neurospora crassa encodes a trans-acting regulatory protein that activates the expression of a number of structural genes which code for nitrogen catabolic enzymes, including nitrate reductase. The NIT2 protein contains a Cys2/Cys2-type zinc-finger DNA-binding domain that recognizes promoter regions of the Neurospora nitrogen-related genes. The NIT2 zinc-finger domain/beta-Gal fusion protein was shown to recognize and bind in a specific manner to two upstream fragments of the nia gene of Lycopersicon esculentum (tomato) in vitro, whereas two mutant NIT2 proteins failed to bind to the same fragments. The dissociation kinetics of the complexes formed between the NIT2 protein and the Neurospora nit-3 and the tomato nia gene promoters were examined; NIT2 binds more strongly to the nit-3 promoter DNA fragment than it does to fragments derived from the plant nitrate reductase gene itself. The observed specificity of the binding suggests the existence of a NIT2-like homolog which regulates the expression of the nitrate assimilation pathway of higher plants. |
| تدمد: | 0172-8083 1432-0983 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::656802f73cf20df0f5ca6ccc70bff209Test https://pubmed.ncbi.nlm.nih.gov/1531184Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....656802f73cf20df0f5ca6ccc70bff209 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01728083 14320983 |
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