Genetic analysis of the yeast NUD1 endo-exonuclease: a role in the repair of DNA double-strand breaks
| العنوان: | Genetic analysis of the yeast NUD1 endo-exonuclease: a role in the repair of DNA double-strand breaks |
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| المؤلفون: | Paul Kauler, Shirley Lehnert, Terry Y-K Chow, Benyam Asefa, Denis Cournoyer |
| المصدر: | Current Genetics. 34:360-367 |
| بيانات النشر: | Springer Science and Business Media LLC, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Saccharomyces cerevisiae Proteins, DNA Repair, DNA damage, DNA repair, genetic processes, Saccharomyces cerevisiae, Biology, Gene Expression Regulation, Enzymologic, Homology directed repair, chemistry.chemical_compound, Gene Expression Regulation, Fungal, Genetics, Deoxyribonucleases, Type II Site-Specific, tRNA Methyltransferases, Deoxyribonucleases, fungi, DNA, General Medicine, DNA repair protein XRCC4, biology.organism_classification, Molecular biology, Homologous Recombination Pathway, Non-homologous end joining, enzymes and coenzymes (carbohydrates), Phenotype, chemistry, DNA Damage |
| الوصف: | The deoxyribonucleases (DNases) have been shown genetically to be important in the vital processes of DNA repair and recombination. The NUD1 gene, which codes for an endo-exonuclease of Saccharomyces cerevisiae, was analyzed for its role in the DNA double-strand break (DSB) repair processes. While the nud1 strain is only slightly sensitive to ionizing radiation, expression of the HO-endonuclease to introduce a DSB at the MAT locus in that strain results in cell death. Cell survival is inversely proportional to the duration of HO-endonuclease expression. Analysis of the surviving colonies from the nud1 strain indicated that many of the survivors are sterile and that the proportion of these sterile survivors increases with the time of HO-endonuclease expression. On the other hand, the surviving colonies from the isogenic NUD1 strain are mating-proficient. Interestingly, double mutants of nud1 rad52 are more resistant to ionizing irradiation than the rad52 strain and have a cell-survival fraction of 32% for rad52-1 nud1 and 9% for rad52::URA3 nud1 following prolonged HO-endonuclease expression, indicating that nud1 has a suppressor effect on the DSB-induced lethality in rad52. Polymerase chain reaction analysis showed that many of the nud1 survivors contained small alterations within theMAT locus, suggesting that the survivors arose through the process of non-homologous end-joining. These results suggest that the endo-exonuclease acts at a DSB to promote DNA repair via the homologous recombination pathway. |
| تدمد: | 1432-0983 0172-8083 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d857700de4cf4adf2d2aae7eb03113eTest https://doi.org/10.1007/s002940050407Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....9d857700de4cf4adf2d2aae7eb03113e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320983 01728083 |
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