Isolation of High Molecular Length DNA: Alfalfa, Pea, Rice, Sorghum, Soybean, and Spinach
| العنوان: | Isolation of High Molecular Length DNA: Alfalfa, Pea, Rice, Sorghum, Soybean, and Spinach |
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| المؤلفون: | Megumi Hada, John C. Sutherland, Paula V. Bennett, L.C. Pope, A.M. Lepre, Shinnosuke Takayanagi, F.E. Quaite, Jun Hidema, Joe H. Sullivan, Betsy M. Sutherland |
| المصدر: | Crop Science. 41:167-172 |
| بيانات النشر: | Wiley, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Spinacia, Oryza sativa, DNA damage, fungi, food and beverages, Pyrimidine dimer, Biology, biology.organism_classification, DNA extraction, chemistry.chemical_compound, Biochemistry, chemistry, Botany, Spinach, Medicago sativa, Agronomy and Crop Science, DNA |
| الوصف: | Measuring DNA damage in higher plants is important in assessing the impaets of environmental conditions, e.g., increased UV resulting from ozone depletion, and in testing the relationship of productivity to DNA damage and repair. Sunlight exposure of plants produces UV induced DNA damages measurable by treating DNA with damage-specific enzymes and dispersion of DNA molecules in denaturing media. Such DNA must be enzyme-digestible, with few single strand breaks. DNA isolation must preclude repair, providing a snapshot of DNA damage. We developed a method for isolating DNA from several crop plants, both monocols and dicots-alfalfa (Medicago sativa L.), pea (Pisam sativam L.), rice (Oryza sativa L.), soybean [Glycine max (L.) Merr.], sorghum [Sorghum bicolor (L.) Moench], and spinach (Spinacia oleraceae L.). This method is simple, readily deals with multiple samples, and avoids organic solvents. We show that pyrimidine dimers can readily be quantified in DNA prepared by this method. This method should also be useful for other experiments requiring high molecular length, enzymatically digestible plant DNA. |
| تدمد: | 1435-0653 0011-183X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::8fa082be4ffa0480ab4f7ce5275fdd01Test https://doi.org/10.2135/cropsci2001.411167xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........8fa082be4ffa0480ab4f7ce5275fdd01 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14350653 0011183X |
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