TROSY effects in MAS solid-state NMR
| العنوان: | TROSY effects in MAS solid-state NMR |
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| المؤلفون: | Veniamin Chevelkov, Bernd Reif |
| المصدر: | Concepts in Magnetic Resonance Part A. :143-156 |
| بيانات النشر: | Wiley, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Nuclear magnetic resonance, Solid-state nuclear magnetic resonance, Carbon-13 NMR satellite, Chemistry, Relaxation (NMR), Magic angle spinning, Resonance, Transverse relaxation-optimized spectroscopy, Nuclear magnetic resonance spectroscopy, Spectroscopy |
| الوصف: | Use of transverse relaxation-optimized spectroscopy (TROSY) type techniques had a dramatic impact on the study of large proteins with a molecular weight >30kDa for solution-state NMR. In the solid-state, such an effect would not be expected a prior, as the investigated molecules are immobilized. However, local motions induce fluctuations of the local fields experienced by the nuclear spins and, this way, are effective for relaxation. We demonstrate that protein dynamics can significantly influence the resonance line width in ultra high resolution MAS (magic angle spinning) solid-state NMR experiments. Averaging of the 15NHα/β multiplet components as a consequence of 1H decoupling induces effective broadening of the 15N resonance. Application of TROSY type techniques that select only the narrow component of the multiplet pattern results in an increased resolution and, thus, will be of benefit for MAS solid-state NMR spectroscopy. © 2008 Wiley Periodicals, Inc. Concepts Magn Reson Part A 32A: 143–156, 2008. |
| تدمد: | 1552-5023 1546-6086 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::7f1bac143f5869d312b77d39024e3b7cTest https://doi.org/10.1002/cmr.a.20106Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........7f1bac143f5869d312b77d39024e3b7c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15525023 15466086 |
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