DNA conformational dynamics: approach to the physical mapping of genome
| العنوان: | DNA conformational dynamics: approach to the physical mapping of genome |
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| المؤلفون: | Mikhail Viktorovich Golovkin, R. V. Polozov, Dmitrii Yurievich Nechipurenko, Yurii Dmitrievich Nechipurenko, Il'icheva Ia, S. L. Grokhovsky, L. A. Panchenko |
| المصدر: | Компьютерные исследования и моделирование, Vol 2, Iss 4, Pp 419-428 (2010) |
| بيانات النشر: | Izhevsk Institute of Computer Science, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | lcsh:T57-57.97, lcsh:Mathematics, sequence effects on DNA structure, Dynamics (mechanics), Computational biology, Biology, lcsh:QA1-939, Genome, Computer Science Applications, d(CpG) dinucleotide, chemistry.chemical_compound, Computational Theory and Mathematics, chemistry, Modeling and Simulation, lcsh:Applied mathematics. Quantitative methods, DNA flexibility, ultrasonic cleavage of DNA, Physical mapping, DNA |
| الوصف: | Recently we have developed a new method for studying DNA based on ultrasound - induced cleavage of DNA sugar-phosphate backbone. Relative cleavage rates of the phosphodiester bonds in all 16 dinucleotides have been determined. The increased amount of data sampling (of more than 20 000 nucleotides) made it also possible to obtain cleavage rates in all 256 possible tetranucleotides. These values quantitatively characterize sequence effects on conformational dynamics of DNA sugar phosphate backbone. Same type of DNA heterogeneity have been discovered and studied using its chemical cleavage induced by various chemical agents and DNAse I. The presence of essential heterogeneity in structural properties of DNA might be a key for physical mapping of the genomes, i.e. determining the structural profiles being responsible for DNA recognition by gene expression regulation machinery. |
| تدمد: | 2077-6853 2076-7633 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f647d93c7ee9469b624925b4ee0a7f6Test https://doi.org/10.20537/2076-7633-2010-2-4-419-428Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1f647d93c7ee9469b624925b4ee0a7f6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20776853 20767633 |
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