التفاصيل البيبلوغرافية
| العنوان: |
Live slow-frozen human tumor tissues viable for 2D, 3D, ex vivo cultures and single-cell RNAseq. |
| المؤلفون: |
Restivo, Gaetana, Tastanova, Aizhan, Balázs, Zsolt, Panebianco, Federica, Diepenbruck, Maren, Ercan, Caner, Preca, Bodgan-T., Hafner, Jürg, Weber, Walter P., Kurzeder, Christian, Vetter, Marcus, Soysal, Simone Münst, Beisel, Christian, Bentires-Alj, Mohamed, Piscuoglio, Salvatore, Krauthammer, Michael, Levesque, Mitchell P. |
| المصدر: |
Communications Biology; 10/28/2022, Vol. 5 Issue 1, p1-12, 12p |
| مصطلحات موضوعية: |
RNA sequencing, TRANSLATIONAL research, CANCER research, HUMAN beings, TISSUES |
| مستخلص: |
Biobanking of surplus human healthy and disease-derived tissues is essential for diagnostics and translational research. An enormous amount of formalin-fixed and paraffin-embedded (FFPE), Tissue-Tek OCT embedded or snap-frozen tissues are preserved in many biobanks worldwide and have been the basis of translational studies. However, their usage is limited to assays that do not require viable cells. The access to intact and viable human material is a prerequisite for translational validation of basic research, for novel therapeutic target discovery, and functional testing. Here we show that surplus tissues from multiple solid human cancers directly slow-frozen after resection can subsequently be used for different types of methods including the establishment of 2D, 3D, and ex vivo cultures as well as single-cell RNA sequencing with similar results when compared to freshly analyzed material. Fresh vs. slow-frozen tissues from various malignancies are compared for the establishment of 2D, 3D and ex vivo cultures, as well as for scRNAseq analysis, and found to be comparable and suitable for cancer research. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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