Identification of a transcription factor, PunR, that regulates the purine and purine nucleoside transporter punC in E. coli
| العنوان: | Identification of a transcription factor, PunR, that regulates the purine and purine nucleoside transporter punC in E. coli |
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| المؤلفون: | Anand V. Sastry, Ye Gao, Hyun Gyu Lim, Ying Hefner, Dmitry A. Rodionov, Bernhard O. Palsson, Irina A. Rodionova, Milton H. Saier |
| المصدر: | Communications Biology, Vol 4, Iss 1, Pp 1-10 (2021) Communications Biology Communications biology, vol 4, iss 1 Rodionova, I A, Gao, Y, Sastry, A, Hefner, Y, Lim, H G, Rodionov, D A, Saier, M H & Palsson, B O 2021, ' Identification of a transcription factor, PunR, that regulates the purine and purine nucleoside transporter punC in E. coli ', Communications Biology, vol. 4, no. 1, 991 . https://doi.org/10.1038/s42003-021-02516-0Test |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Purine, QH301-705.5, 1.1 Normal biological development and functioning, Mutant, Medicine (miscellaneous), Guanosine, Nucleoside Transport Proteins, Nucleoside transporter, Article, General Biochemistry, Genetics and Molecular Biology, Cell growth, chemistry.chemical_compound, Underpinning research, Bacterial genetics, Genetics, Escherichia coli, medicine, Biology (General), Inosine, Data mining, Gene, Bacterial systems biology, biology, Escherichia coli Proteins, Membrane Transport Proteins, Biological Transport, Transporter, Adenosine, Cell biology, Infectious Diseases, chemistry, Purines, biology.protein, Infection, General Agricultural and Biological Sciences, Biotechnology, Transcription Factors, medicine.drug |
| الوصف: | Many genes in bacterial genomes are of unknown function, often referred to as y-genes. Recently, the analytic methods have divided bacterial transcriptomes into independently modulated sets of genes (iModulons). Functionally annotated iModulons that contain y-genes lead to testable hypotheses to elucidate y-gene function. The inversely correlated expression of a putative transporter gene, ydhC, relative to purine biosynthetic genes, has led to the hypothesis that it encodes a purine-related transporter and revealed a LysR-family regulator, YdhB, with a predicted 23-bp palindromic binding motif. RNA-Seq analysis of a ydhB knockout mutant confirmed the YdhB-dependent activation of ydhC in the presence of adenosine. The deletion of either the ydhC or the ydhB gene led to a substantially decreased growth rate for E. coli in minimal medium with adenosine, inosine, or guanosine as the nitrogen source. Taken together, we provide clear evidence that YdhB activates the expression of the ydhC gene that encodes a purine transporter in E. coli. We propose that the genes ydhB and ydhC be re-named as punR and punC, respectively. Rodionova et al. find that the putative transporter gene, ydhC and its regulator ydhB are involved in purine transportation and that the expression of the ydhC gene is activated by the YdhB in E. coli. The authors suggest renaming the regulator PunR and the transporter PunC, respectively. |
| وصف الملف: | application/pdf |
| تدمد: | 2399-3642 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::735deba44e153ee1061ca93c3c00341dTest https://doi.org/10.1038/s42003-021-02516-0Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....735deba44e153ee1061ca93c3c00341d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23993642 |
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