Preparation of Meiotic Chromosomes from Larval and Pupal Drosophila Testes
| العنوان: | Preparation of Meiotic Chromosomes from Larval and Pupal Drosophila Testes |
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| المؤلفون: | Maurizio Gatti, Giovanni Cenci, Silvia Bonaccorsi, Maria Grazia Giansanti |
| المصدر: | Cold Spring Harbor Protocols. 2011:prot5579 |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | 6 diamidino 2 phenylindole, acetoorcein, Male, 4 [5 (4 methyl 1 piperazinyl)[2, 4', 5' bi 1h benzimidazol] 2' yl]phenol, article, chromosome preparation, drosophila, in situ hybridization, larval stage, meiosis, nonhuman, pupa (life cycle stage), reagent, staining, testis, unclassified drug, Cytological Techniques, General Biochemistry, Genetics and Molecular Biology, Specimen Handling, Meiosis, Spermatocytes, Testis, Animals, Drosophila (subgenus), Larva, biology, fungi, Pupa, biology.organism_classification, Chromosomes, Insect, Cell biology, Drosophila |
| الوصف: | INTRODUCTIONSeveral techniques have been developed for the preparation of Drosophila male meiotic chromosomes. All of these techniques allow a clear visualization of chromosomes, but not of other cellular structures such as the cytoskeleton and the spindle. This protocol describes two methods for preparation of meiotic chromosomes from larval and pupal testes. The first is a very simple method for preparing aceto-orcein-stained chromosomes. The second allows preparation of unstained chromosomes that can subsequently be stained with various dyes such as Giemsa, Hoechst 33258, and DAPI, or processed for in situ hybridization. |
| تدمد: | 1559-6095 1940-3402 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::272a4886b045ccdb725a6843f2fcb397Test https://doi.org/10.1101/pdb.prot5579Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....272a4886b045ccdb725a6843f2fcb397 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15596095 19403402 |
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