Cell-free microRNAs as early predictors of graft viability during ex vivo normothermic machine perfusion of human donor livers
| العنوان: | Cell-free microRNAs as early predictors of graft viability during ex vivo normothermic machine perfusion of human donor livers |
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| المؤلفون: | Jasmijn W. Selten, Robert J. Porte, Henk P. Roest, Jan N. M. IJzermans, Vincent E de Meijer, Luc J. W. van der Laan, Jeroen de Jonge, Alix P M Matton |
| المساهمون: | Surgery, Groningen Institute for Organ Transplantation (GIOT), Center for Liver, Digestive and Metabolic Diseases (CLDM) |
| المصدر: | Clinical Transplantation Clinical Transplantation, 34. Wiley-Blackwell Publishing Ltd Clinical Transplantation, 34(3):13790. Wiley |
| بيانات النشر: | Wiley-Blackwell Publishing Ltd, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Bilirubin, BIOMARKERS, hepatocyte-derived microRNA, Cold storage, 030230 surgery, Cholangiocyte, SERUM, Andrology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, MiR-122, Living Donors, INJURY, Medicine, Humans, CRITERIA, hepatocyte‐derived microRNA, Circulating MicroRNA, PRESERVATION, cholangiocyte‐derived microRNA, RELEASE, Machine perfusion, business.industry, TRANSPLANTATION, Original Articles, Organ Preservation, miR-222, Liver Transplantation, miR-122, Transplantation, Perfusion, cholangiocyte-derived microRNA, medicine.anatomical_structure, miR‐222, chemistry, miR‐122, Liver, Hepatocyte, biomarker, 030211 gastroenterology & hepatology, Original Article, business, Ex vivo |
| الوصف: | Background Cell-free microRNAs (miRs) have emerged as early and sensitive biomarkers for tissue injury and function. This study aimed to investigate whether the release of hepatocyte-derived microRNAs (HDmiRs) and cholangiocyte-derived miRs (CDmiRs) correlates with hepato-cholangiocellular injury and function during oxygenated, normothermic machine perfusion (NMP) of human liver grafts.Methods Donor livers (n = 12), declined for transplantation, were subjected to oxygenated NMP (6 hours) after a period of static cold storage (median 544 minutes (IQR 421-674)). Perfusate and bile samples were analyzed by qRT-PCR for HDmiR-122 and CDmiR-222. Spearman correlations were performed between miR levels and currently available indicators and classic markers.Results Both HDmiR-122 and CDmiR-222 levels in perfusate at 30 minutes of NMP strongly correlated with hepatocyte injury (peak perfusate AST) and cholangiocyte injury (peak biliary LDH). In bile, only CDmiR-222 correlated with these injury markers. For hepato-cholangiocellular function, both miRs in perfusate correlated with total bilirubin, while HDmiR-122 (in perfusate) and CDmiR-222 (in bile) correlated with bicarbonate secretion. Both the relative ratio of HDmiR-122/CDmiR-222 and AST in perfusate at 30 minutes significantly correlated with cumulative bile production, but only the relative ratio was predictive of histopathological injury after 6 hours NMP.Conclusion Early levels of HDmiR-122 and CDmiR-222, in perfusate and/or bile, are predictive of excretory functions and hepato-cholangiocellular injury after 6 hours NMP. These miRs may represent new biomarkers for graft viability and function during machine perfusion. |
| وصف الملف: | application/pdf |
| تدمد: | 1399-0012 0902-0063 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04ea7f0bc8a15d426d0fe0af6f52a1e5Test https://doi.org/10.1111/ctr.13790Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....04ea7f0bc8a15d426d0fe0af6f52a1e5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13990012 09020063 |
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