دورية أكاديمية
Cell-free microRNAs as early predictors of graft viability during ex vivo normothermic machine perfusion of human donor livers
| العنوان: | Cell-free microRNAs as early predictors of graft viability during ex vivo normothermic machine perfusion of human donor livers |
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| المؤلفون: | Matton, A.P.M. (Alix P. M.), Selten, J.W. (Jasmijn W.), Roest, H.P. (Henk), Jonge, J. (Jeroen) de, IJzermans, J.N.M. (Jan), Meijer, V.E. (Vincent) de, Porte, R.J. (Robert), Laan, L.J.W. (Luc) van der |
| المصدر: | Clinical Transplantation |
| سنة النشر: | 2020 |
| المجموعة: | RePub - Publications from Erasmus University, Rotterdam |
| مصطلحات موضوعية: | biomarker, cholangiocyte-derived microRNA, hepatocyte-derived microRNA, miR-122, miR-222, transplantation |
| الوصف: | Background: Cell-free microRNAs (miRs) have emerged as early and sensitive biomarkers for tissue injury and function. This study aimed to investigate whether the release of hepatocyte-derived microRNAs (HDmiRs) and cholangiocyte-derived miRs (CDmiRs) correlates with hepato-cholangiocellular injury and function during oxygenated, normothermic machine perfusion (NMP) of human liver grafts. Methods: Donor livers (n = 12), declined for transplantation, were subjected to oxygenated NMP (6 hours) after a period of static cold storage (median 544 minutes (IQR 421-674)). Perfusate and bile samples were analyzed by qRT-PCR for HDmiR-122 and CDmiR-222. Spearman correlations were performed between miR levels and currently available indicators and classic markers. Results: Both HDmiR-122 and CDmiR-222 levels in perfusate at 30 minutes of NMP strongly correlated with hepatocyte injury (peak perfusate AST) and cholangiocyte injury (peak biliary LDH). In bile, only CDmiR-222 correlated with these injury markers. For hepato-cholangiocellular function, both miRs in perfusate correlated with total bilirubin, while HDmiR-122 (in perfusate) and CDmiR-222 (in bile) correlated with bicarbonate secretion. Both the relative ratio of HDmiR-122/CDmiR-222 and AST in perfusate at 30 minutes significantly correlated with cumulative bile production, but only the relative ratio was predictive of histopathological injury after 6 hours NMP. Conclusion: Early levels of HDmiR-122 and CDmiR-222, in perfusate and/or bile, are predictive of excretory functions and hepato-cholangiocellular injury after 6 hours NMP. These miRs may represent new biomarkers for graft viability and function during machine perfusion. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://repub.eur.nl/pub/125111Test; urn:hdl:1765/125111 |
| DOI: | 10.1111/ctr.13790 |
| الإتاحة: | https://doi.org/10.1111/ctr.13790Test http://repub.eur.nl/pub/125111Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.7AAB63B7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/ctr.13790 |
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