دورية أكاديمية
Glycan biomarkers of autoimmunity and bile acid-associated alterations of the human glycome: primary biliary cirrhosis and primary sclerosing cholangitis-specific glycans
| العنوان: | Glycan biomarkers of autoimmunity and bile acid-associated alterations of the human glycome: primary biliary cirrhosis and primary sclerosing cholangitis-specific glycans |
|---|---|
| المؤلفون: | Maverakis, E., Merleev, A.A., Park, D., Kailemia, M.J., Xu, G.G., Ruhaak, L.R., Kim, K., Hong, Q.T., Li, Q.Y., Leung, P., Liakos, W., Wan, Y.J.Y., Bowlus, C.L., Marusina, A.I., Lal, N.N., Xie, Y.X., Luxardi, G., Lebrilla, C.B. |
| المصدر: | Clinical Immunology |
| سنة النشر: | 2021 |
| المجموعة: | Leiden Repository (Leiden University) |
| مصطلحات موضوعية: | N-glycopeptides, Multiple Reacton Monitoring, plasma, biomarker testing, Primary Biliary Cirrhosis (PBC), Primary Sclerosing Cholangitis (PSC) |
| الوصف: | We have recently introduced multiple reaction monitoring (MRM) mass spectrometry as a novel tool for glycan biomarker research and discovery. Herein, we employ this technique to characterize the site-specific glycan alterations associated with primary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC). Glycopeptides associated with disease severity were also identified. Multinomial regression modelling was employed to construct and validate multi-analyte diagnostic models capable of accurately distinguishing PBC, PSC, and healthy controls from one another (AUC = 0.93 +/- 0.03). Finally, to investigate how disease-relevant environmental factors can influence glycosylation, we characterized the ability of bile acids known to be differentially expressed in PBC to alter glycosylation. We hypothesize that this could be a mechanism by which altered selfantigens are generated and become targets for immune attack. This work demonstrates the utility of the MRM method to identify diagnostic site-specific glycan classifiers capable of distinguishing even related autoimmune diseases from one another. ; Afdeling Klinische Chemie en Laboratoriumgeneeskunde (AKCL) |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://www.sciencedirect.com/science/article/pii/S1521661621001625?via%3DihubTest; lumc-id: 120524458; https://hdl.handle.net/1887/3212858Test |
| DOI: | 10.1016/j.clim.2021.108825 |
| الإتاحة: | https://doi.org/10.1016/j.clim.2021.108825Test https://hdl.handle.net/1887/3212858Test https://www.sciencedirect.com/science/article/pii/S1521661621001625?via%3DihubTest |
| رقم الانضمام: | edsbas.7CE1F296 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.clim.2021.108825 |
|---|