IgE cross-reactivity measurement of cashew nut, hazelnut and peanut using a novel IMMULITE inhibition method
| العنوان: | IgE cross-reactivity measurement of cashew nut, hazelnut and peanut using a novel IMMULITE inhibition method |
|---|---|
| المؤلفون: | Roy Gerth van Wijk, Nasrin Aazamy, Nicolette W. de Jong, Huub F. J. Savelkoul, Manou R. Batstra, Harry J. Wichers, Shanna Bastiaan-Net, Marco W.J. Schreurs, Johanna P. M. van der Valk |
| المساهمون: | Internal Medicine, Immunology |
| المصدر: | Clinical Chemistry and Laboratory Medicine 58 (2020) 11 Clinical Chemistry and Laboratory Medicine, 58(11), 1875-1883 Clinical Chemistry and Laboratory Medicine, 58(11), 1875-1883. De Gruyter |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Allergy, Arachis, Clinical Biochemistry, medicine.disease_cause, Immunoglobulin E, Cross-reactivity, 0302 clinical medicine, Allergen, hazelnut, Food science, Cashew nut, Child, Betula, Immunoassay, biology, Food Chemistry, digestive, oral, and skin physiology, food and beverages, General Medicine, cashew nut, Health & Consumer Research, Pollen, Nut Hypersensitivity, allergy diagnostics, Celbiologie en Immunologie, Cross Reactions, 03 medical and health sciences, Corylus, IMMULITE technology, Levensmiddelenchemie, medicine, Potency, Humans, Anacardium, Peanut Hypersensitivity, Food components, Food, Health & Consumer Research, VLAG, Biochemistry (medical), Allergens, medicine.disease, Birch pollen, 030104 developmental biology, 030228 respiratory system, Cell Biology and Immunology, IgE cross-reactivity, Food, biology.protein, WIAS, peanut |
| الوصف: | Background Tree nut-allergic individuals are often sensitised towards multiple nuts and seeds. The underlying cause behind a multi-sensitisation for cashew nut, hazelnut, peanut and birch pollen is not always clear. We investigated whether immunoglobulin E antibody (IgE) cross-reactivity between cashew nut, hazelnut and peanut proteins exists in children who are multi-allergic to these foods using a novel IMMULITE®-based inhibition methodology, and investigated which allergens might be responsible. In addition, we explored if an allergy to birch pollen might play a role in this co-sensitisation for cashew nut, hazelnut and peanut. Methods Serum of five children with a confirmed cashew nut allergy and suffering from allergic symptoms after eating peanut and hazelnut were subjected to inhibition immunoassays using the IMMULITE® 2000 XPi. Serum-specific IgE (sIgE) to seed storage allergens and pathogenesis-related protein 10 (PR10) allergens were determined and used for molecular multicomponent allergen correlation analyses with observed clinical symptoms and obtained inhibition data. Results IgE cross-reactivity was observed in all patients. Hazelnut extract was a strong inhibitor of cashew nut sIgE (46.8%), while cashew nut extract was less able to inhibit hazelnut extract (22.8%). Peanut extract showed the least inhibition potency. Moreover, there are strong indications that a birch pollen sensitisation to Bet v 1 might play a role in the observed symptoms provoked upon ingestion of cashew nut and hazelnut. Conclusions By applying an adjusted working protocol, the IMMULITE® technology can be used to perform inhibition assays to determine the risk of sIgE cross-reactivity between very different food components. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1434-6621 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bbe10efec856880be91678334a06323Test https://doi.org/10.1515/cclm-2019-1083Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1bbe10efec856880be91678334a06323 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14346621 |
|---|