The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020
| العنوان: | The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020 |
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| المؤلفون: | Huggett, J. F., Whale, A. S., Spiegelaere, W. De, Trypsteen, W., Nour, A. A., Bae, Y.-K., Benes, V., Burke, D. G., Corbisier, Philippe, Cleveland, Megan, Devonshire, Alison S, Dong, Lianhua, Drandi, Deniela, Foy, Carole, Garson, J. A., Hellemans, J., He, H.-J., Kubista, M., Lievens, A., Makrigiorgos, M., Milavec, M., Mueller, R. D., Nolan, T., O'Sullivan, D., Pfaffl, M. W., Roediger, S., Romsos, E., Shipley, G., Taly, V., Untergasser, A., Wittwer, C., Bustin, S., Vandesompele., And J. |
| المساهمون: | University of Surrey (UNIS), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université de Paris (UP), dMIQE Group: Alexandra S Whale, Ward De Spiegelaere, Wim Trypsteen, Afif Abdel Nour, Young-Kyung Bae, Vladimir Benes, Daniel Burke, Megan Cleveland, Philippe Corbisier, Alison S Devonshire, Lianhua Dong, Daniela Drandi, Carole A Foy, Jeremy A Garson, Hua-Jun He, Jan Hellemans, Mikael Kubista, Antoon Lievens, Mike G Makrigiorgos, Mojca Milavec, Reinhold D Mueller, Tania Nolan, Denise M O'Sullivan, Michael W Pfaffl, Stefan Rödiger, Erica L Romsos, Gregory L Shipley, Valerie Taly, Andreas Untergasser, Carl T Wittwer, Stephen A Bustin, Jo Vandesompele, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), TALY, Valerie |
| المصدر: | CLINICAL CHEMISTRY Clinical Chemistry Clinical Chemistry, American Association for Clinical Chemistry, 2020, 66 (8), pp.1012-1029. ⟨10.1093/clinchem/hvaa125⟩ |
| بيانات النشر: | Oxford University Press (OUP), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, POLYMERASE-CHAIN-REACTION, Computer science, Process (engineering), [SDV]Life Sciences [q-bio], EGFR, Absolute quantification, ABSOLUTE QUANTIFICATION, Clinical Biochemistry, VOLUME VARIABILITY, Guidelines as Topic, Polymerase Chain Reaction, MIQE, 03 medical and health sciences, dMIQE, dPCR, digital PCR, Humans, Nucleic Acids, Publications, 0302 clinical medicine, Software, Digital polymerase chain reaction, Instrumentation (computer programming), PLASMA, business.industry, DROPLET, Biochemistry (medical), Biology and Life Sciences, AMPLIFICATION, DNA COPY NUMBER, Data science, ddc, QPCR, [SDV] Life Sciences [q-bio], 030104 developmental biology, 030220 oncology & carcinogenesis, Key (cryptography), Table (database), business, RESISTANCE |
| الوصف: | Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology. |
| وصف الملف: | application/pdf |
| تدمد: | 1530-8561 0009-9147 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18bb2dfcbc72a2bf3baf77c984c4a96dTest https://doi.org/10.1093/clinchem/hvaa125Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....18bb2dfcbc72a2bf3baf77c984c4a96d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15308561 00099147 |
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