Dietary Rice Bran Protects against Rotavirus Diarrhea and Promotes Th1-Type Immune Responses to Human Rotavirus Vaccine in Gnotobiotic Pigs
| العنوان: | Dietary Rice Bran Protects against Rotavirus Diarrhea and Promotes Th1-Type Immune Responses to Human Rotavirus Vaccine in Gnotobiotic Pigs |
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| المؤلفون: | Ke Wen, Guohua Li, Christine M. Tin, Kevin D. Pelzer, Xingdong Yang, Lijuan Yuan, Haifeng Wang, Jacob Kocher, Elizabeth P. Ryan |
| المصدر: | Clinical and Vaccine Immunology. 21:1396-1403 |
| بيانات النشر: | American Society for Microbiology, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, Diarrhea, Dietary Fiber, Microbiology (medical), Immunoglobulin A, Swine, medicine.medical_treatment, Clinical Biochemistry, Immunology, CD8-Positive T-Lymphocytes, Biology, Antibodies, Viral, Vaccines, Attenuated, medicine.disease_cause, Rotavirus Infections, Immunoglobulin G, Microbiology, Immune system, Adjuvants, Immunologic, Rotavirus, medicine, Animals, Germ-Free Life, Immunology and Allergy, Vaccines, B-Lymphocytes, Immunogenicity, Rotavirus Vaccines, Antibody titer, Oryza, Th1 Cells, Diet, Virus Shedding, biology.protein, medicine.symptom, Adjuvant |
| الوصف: | Rice bran (RB) contains a distinct stoichiometry of phytochemicals that can promote gut mucosal immune responses against enteric pathogens. The effects of RB on rotavirus diarrhea and immunogenicity of an attenuated human rotavirus (HRV) vaccine were evaluated in gnotobiotic pigs. The four treatment groups studied were RB plus vaccine, vaccine only, RB only, and mock control. Pigs in the RB groups were fed the amount of RB that replaced 10% of the pigs' total daily calorie intake from milk starting from 5 days of age until they were euthanized. Pigs in the vaccine groups were orally inoculated with two doses of the attenuated HRV vaccine. A subset of pigs from each group was orally challenged with the homologous virulent HRV on postinoculation day 28. Diarrhea and virus shedding were monitored daily from postchallenge day 0 to day 7. RB feeding significantly protected against diarrhea upon virulent HRV challenge and enhanced the protective rate of the vaccine against rotavirus diarrhea. Consistent with protection, RB significantly increased gamma interferon (IFN-γ)-producing CD4 + and CD8 + T cell responses in intestinal and systemic lymphoid tissues. Furthermore, RB also increased the number of total IgM- and IgA-secreting cells, total serum IgM, IgG, and IgA titers, and HRV-specific IgA titers in intestinal contents. RB reduced the numbers of intestinal and systemic HRV-specific IgA and IgG antibody-secreting cells and reduced serum HRV-specific IgA and IgG antibody titers before the challenge. These results demonstrate clear beneficial effects of RB in protection against rotavirus diarrhea and stimulation of nonspecific and HRV-specific immune responses, as well as its biased Th1-type adjuvant effect for the vaccine. |
| تدمد: | 1556-679X 1556-6811 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0beb02c27a64d99d83ea9bec72d35f02Test https://doi.org/10.1128/cvi.00210-14Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0beb02c27a64d99d83ea9bec72d35f02 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1556679X 15566811 |
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