In vitro anti-HBs antibody synthesis from anti-hepatitis B vaccine recipients
| العنوان: | In vitro anti-HBs antibody synthesis from anti-hepatitis B vaccine recipients |
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| المؤلفون: | Barnaba V, Levrero M, Giovina RUBERTI, van Dyke A, Perrone A, Musca A, Balsano F |
| المصدر: | Scopus-Elsevier Europe PubMed Central |
| سنة النشر: | 1987 |
| مصطلحات موضوعية: | Viral Hepatitis Vaccines, B-Lymphocytes, Hepatitis B Surface Antigens, Pokeweed Mitogens, T-Lymphocytes, Dose-Response Relationship, Immunologic, virus diseases, Humans, chemical and pharmacologic phenomena, Hepatitis B Antibodies, Lymphocyte Culture Test, Mixed, digestive system diseases, Research Article |
| الوصف: | Peripheral blood mononuclear cells (PBMC) from 'responders' recently boosted with hepatitis B vaccine, were studied for synthesis in vitro of antibody to hepatitis B surface antigen (anti-HBs Ab) when stimulated with pokeweed mitogen (PWM) or HBsAg. HBsAg alone can induce an antigen-specific anti-HBs Ab response in vitro; this antibody synthesis is T cell-dependent. In some responders both allogeneic T4+ cells (in absence of PWM or HBsAg) and mixed leucocyte culture supernatants (MLC/SN) (without T cells and antigen) can help responder B cells to produce anti-HBs Ab. Thus, in some immunized subjects, B lymphocytes involved in anti-HBs Ab synthesis are in an advanced phase of differentiation and require only non-antigen specific T cell signals (B cell growth factor or B cell differentiation factor or interleukin 2, etc) to differentiate into antibody-secreting cells. Moreover, the concentration of the antigen necessary to suppress anti-HBs Ab production induced by HBsAg was five times lower than that necessary to suppress antibody production induced by PWM. T cell help for antigen induced anti-HBs Ab could be different from T cell help for the PWM-induced anti-HBs Ab response. Moreover, the finding that the low HBsAg doses inhibiting specific response did not affect the PWM-driven anti-HBs response suggests that antigen-specific T suppressor cells could play a role in this context. |
| تدمد: | 0009-9104 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::cca494061e4f2e00243eb5d53db7af90Test https://pubmed.ncbi.nlm.nih.gov/2962789Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....cca494061e4f2e00243eb5d53db7af90 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00099104 |
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