التفاصيل البيبلوغرافية
| العنوان: |
Two maturation-associated mouse erythrocyte receptors of human B cells. |
| المؤلفون: |
Forbes, I.J., Zalewski, P. D., Valente, L., Gee, D. |
| المصدر: |
Clinical & Experimental Immunology; Feb1982, Vol. 47 Issue 2, p405-413, 9p |
| مصطلحات موضوعية: |
ERYTHROCYTES, BLOOD cells, LYMPHOCYTIC leukemia, LYMPHOCYTES, CHRONIC diseases, CELL membranes |
| مستخلص: |
Trypsin treatment of chronic lymphocytic leukaemia (CLL) cells which have the capacity to rosette with mouse erythrocytes (M), the BM+ subtype, inhibits their capacity to rosette and releases a substance into the supernatant which agglutinates mouse and rat erythrocytes but not erythrocytes of five other species tested. This substance has been named immature B-cell lectin (IBL). The specificity of IBL was further demonstrated by fluorescence labelling, absorption and latex rosetting. IBL does not bind to pronasetreated M (pro M), indicating that it has the specificity of R1 as distinct from R1 which binds lo a pronase-resistant ligand on M. Other evidence that I BL is associated with B-cell membrane receptors for mouse erythrocytes is as follows: (1) The amount of IBL released into the supernatant correlated with the trypsin sensitivity of M resetting with different clones of BM+ CLL cells. (2) Only small amounts of IBL were released from non-rosetting cells (T ceils and mature B cells). (3) Binding properties of IBL were inhibited by extract of M but not extract from ox erythrocytes. (4) High-titre solutions of IBL conferred the capacity to form M rosettes on certain types of non-rosetting B cells. IBL has a dual binding specificity. Its binding to M is inhibited by fetuin and mannan, while its binding to B ceils is not inhibited by these substances. The relationship of IBL to other membrane lectins including fibro nectin is discussed. Preliminary characterization indicates a high-molecular-weight (at least 300,000 daltons) glycoprotein which has a pronounced tendency to aggregate in solution. The relationship of IBL to stages of human B-cell maturation is discussed. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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