المؤلفون: Elena Aloisio, Giulia Colombo, Alberto Dolci, Mauro Panteghini, Claudia Arrigo

المصدر: Clinica Chimica Acta; International Journal of Clinical Chemistry

مصطلحات موضوعية: Clinical Biochemistry, AST, aspartate aminotransferase, Liver injury, Systemic inflammation, Biochemistry, Gastroenterology, law.invention, chemistry.chemical_compound, fluids and secretions, law, polycyclic compounds, Medicine, IL-6, interleukin-6, LDH, lactate dehydrogenase, biology, Liver Diseases, virus diseases, Alanine Transaminase, R2, coefficient of determination, General Medicine, ICU, intensive care unit, Intensive care unit, P-5′-P, pyridoxal-5-phosphate, CRP, C-reactive protein, Alkaline phosphatase, medicine.symptom, medicine.medical_specialty, Aspartate aminotransferase, digestive system, Article, SARS-CoV-2, severe acute respiratory syndrome coronavirus 2 COVID-19. 2019 coronavirus disease, ALT, alanine aminotransferase, Lactate dehydrogenase, Internal medicine, Humans, Clinical significance, IFCC, International Federation of Clinical Chemistry and Laboratory Medicine, Aspartate Aminotransferases, IQR, interquartile range, ALP, alkaline phosphatase, SARS-CoV-2, business.industry, Biochemistry (medical), URL, upper reference limit, COVID-19, medicine.disease, digestive system diseases, OR, odds ratio, CI, confidence interval, HI, hemolysis index, GGT, γ-glutamyltransferase, R, correlation coefficient, Alanine transaminase, chemistry, biology.protein, Creatine kinase, business, CK, creatine kinase

الوصف: Background Aspartate aminotransferase (AST) is often increased in COVID-19 and, in some studies, AST abnormalities were associated with mortality risk. Methods 2054 hospitalized COVID-19 patients were studied. To identify sources of AST release, correlations between AST peak values and other biomarkers of tissue damage, i.e., alanine aminotransferase (ALT) for hepatocellular damage, creatine kinase (CK) for muscle damage, lactate dehydrogenase for multiorgan involvement, alkaline phosphatase and γ-glutamyltransferase for cholestatic injury, and C-reactive protein (CRP) for systemic inflammation, were performed and coefficients of determination estimated. The role of AST to predict death and intensive care unit admission during hospitalization was also evaluated. All measurements were performed using standardized assays. Results AST was increased in 69% of patients. Increases could be fully explained by summing the effects of hepatocellular injury [AST dependence from ALT, 66.8% [95% confidence interval (CI): 64.5–69.1)] and muscle damage [AST dependence from CK, 42.6% (CI: 39.3–45.8)]. We were unable to demonstrate an independent association of AST increases with worse outcomes. Conclusion The mechanisms for abnormal AST in COVID-19 are likely multifactorial and a status related to tissue suffering could play a significant role. The clinical significance of AST elevations remains unclear.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3d1d325846e5aacf52eb8f0ef551a94Test
https://doi.org/10.1016/j.cca.2021.08.012Test

المؤلفون: Li Zhang, Shuomin Li, Zhufeng Chen, Wanju Xu, Ma Wanshan, Hao Mingju, Yuanxun Fang, Shi Xiaohong

المصدر: Clinica Chimica Acta; International Journal of Clinical Chemistry

مصطلحات موضوعية: Review, Disease, TNF-α, tumor necrosis factor-α, Biochemistry, US, United States, IL-1β, interleukin 1β, PCR, polymerase chain reaction, 0302 clinical medicine, Ang I, angiotensin I, DIC, disseminated intravascular coagulation, COVID-19, coronavirus disease 2019, LDH, lactate dehydrogenase, General Medicine, Clinical Laboratory Services, CSS, cytokine storm syndrome, NAAT, nucleic acid amplification testing, RT-LAMP, reverse transcription loop-mediated isothermal amplification, 030220 oncology & carcinogenesis, CRP, C-reactive protein, G-CSF, granulocyte-colony stimulating factor, Viral load, WBC, white blood cell, IgG, immunoglobulin G, ACE2, angiotensin-converting enzyme 2, MCP1, monocyte chemoattractant protein 1, 03 medical and health sciences, RT-PCR, reverse transcription polymerase chain reaction, POCT, point-of-care tests, PT, prothrombin time, Humans, Serologic Tests, RdRp, RNA-dependent RNA polymerase, Risk factor, APTT, activated partial thromboplastin time, ARDS, acute respiratory distress syndrome, IL-2R, interleukin 2R, Aged, Ang II, angiotensin II, LOD, limit of detection, LRT, lower respiratory tract, Ag-RDT, Antigen-detecting rapid diagnostic test, Biomarker, MIP-1α, macrophage inflammatory protein-1α, medicine.disease, SAA, serum amyloid A protein, 030104 developmental biology, Infectious disease (medical specialty), Laboratory diagnosis, Immunology, 0301 basic medicine, PLRs, platelet-to-lymphocyte ratios, Clinical Biochemistry, AST, aspartate aminotransferase, IFN-γ, interferon γ, MERS-CoV, middle east respiratory syndrome coronavirus, CLIA, chemiluminescence-immunoassay, MasR, Mas receptor, TRAIL, TNF related apoptosis inducing ligand, Respiratory disease, BLF, bronchoalveolar lavage fluid, ELISA, enzyme-linked immunosorbent assay, ICU, intensive care unit, VEGF, vascular endothelial growth factor, PCT, procalcitonin, FDA, food and drug administration’s, IL-6, interleukin 6, RBD, receptor binding domains, IP10, induced protein 10, WHO, world health organization, NLR, neutrophil–lymphocyte ratio, Biomarker (medicine), ORF, open-reading frames, medicine.symptom, IL-7, interleukin 7, ACE, angiotensin-converting enzyme, SARS-CoV, severe acute respiratory syndrome coronavirus, Inflammation, IgA, immunoglobulin A, AT2R, AT2 receptor, SARS-CoV-2, severe acute respiratory syndrome coronavirus 2, EUA, emergency use authorization, ALT, alanine aminotransferase, Diabetes mellitus, qRT-PCR, real-time quantitative reverse transcription polymerase chain reaction, IFN-α, interferon α, medicine, FDP, fibrinogen and fibrin degradation products, TMPRSS2, transmembrane protease serine type 2, SARS-CoV-2, business.industry, Biochemistry (medical), COVID-19, RAS, renin-angiotensin system, IgM, immunoglobulin M, PE, pulmonary embolism, IL-2, interleukin 2, IL-10, interleukin 10, Laboratories, business, CK, creatine kinase

الوصف: COVID-19, caused by SARS-CoV-2, is a highly infectious disease, and clinical laboratory detection has played important roles in its diagnosis and in evaluating progression of the disease. Nucleic acid amplification testing or gene sequencing can serve as pathogenic evidence of COVID-19 diagnosing for clinically suspected cases, and dynamic monitoring of specific antibodies (IgM, IgA, and IgG) is an effective complement for false-negative detection of SARS-CoV-2 nucleic acid. Antigen tests to identify SARS-CoV-2 are recommended in the first week of infection, which is associated with high viral loads. Additionally, many clinical laboratory indicators are abnormal as the disease evolves. For example, from moderate to severe and critical cases, leukocytes, neutrophils, and the neutrophil-lymphocyte ratio increase; conversely, lymphocytes decrease progressively but are over activated. LDH, AST, ALT, CK, high-sensitivity troponin I, and urea also increase progressively, and increased D-dimer is an indicator of severe disease and an independent risk factor for death. Severe infection leads to aggravation of inflammation. Inflammatory biomarkers and cytokines, such as CRP, SAA, ferritin, IL-6, and TNF-α, increase gradually. High-risk COVID-19 patients with severe disease, such as the elderly and those with underlying diseases (cardiovascular disease, diabetes, chronic respiratory disease, hypertension, obesity, and cancer), should be monitored dynamically, which will be helpful as an early warning of serious diseases.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7e01a08c176cdc8b4a0445df4480e07Test
https://doi.org/10.1016/j.cca.2021.04.022Test

المؤلفون: Guojun Zhang, Maidinaimu Aibibula, Ning Feng, Huijun Li, Hui Zhao, Jie Lv, Bin Li, Fengming Tian, Xiumin Ma, Liang Wang

المصدر: Clinica Chimica Acta. 510:665-670

مصطلحات موضوعية: 0301 basic medicine, medicine.medical_specialty, Clinical Biochemistry, Disease, Biochemistry, Gastroenterology, Procalcitonin, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, Lactate dehydrogenase, medicine, Humans, In patient, Interleukin 6, L-Lactate Dehydrogenase, biology, Interleukin-6, business.industry, Biochemistry (medical), C-reactive protein, General Medicine, medicine.disease, Systemic inflammatory response syndrome, C-Reactive Protein, 030104 developmental biology, Pancreatitis, chemistry, 030220 oncology & carcinogenesis, Acute Disease, biology.protein, Acute pancreatitis, business, Biomarkers

الوصف: Acute pancreatitis (AP) is an inflammatory disease with rapid progression. In severe cases, it can cause systemic inflammatory response syndrome (SIRS), multiple organ failure (POF) and even death. The study aimed to investigate the diagnostic value of C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6) and lactate dehydrogenase (LDH) in patients with severe AP.AP patients (n = 153) divided into mild AP patients (n = 81) and severe AP patients (n = 72) were selected from June 2014 to June 2016. The demographic information (age, sex) and the hematological parameters (WBC, PLT, CRP, PCT, IL-6, LDH and so on) were analyzed.Significant differences were found out of CRP, PCT, IL-6 and LDH values between AP patients and controls (P 0.05), even those results had significant difference between MAP group and SAP group (P 0.05). In SAP group, the cut-off values of CRP, PCT, IL-6 and LDH were 16.62, 2.29, 16.66, 273.04; sensitivity 55.6%, 77.8%, 80.2%, 82.7%; specificity 73%, 94%, 85%, 96% and AUC 0.637, 0.929, 0.886, 0.919, respectively. The AUC of combined detection of CRP, PCT, IL-6 and LDH was 0.989 (95%CI).The combined detection of CRP, PCT, IL-6 and LDH has a high diagnostic value for judging the severity of AP.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50ccc81012d8efe6161e39ac657aa3cbTest
https://doi.org/10.1016/j.cca.2020.08.029Test

المؤلفون: Giulia Losi, Alessandro Dacrema, Luca Rovero, Andrea Vercelli, Marzia Nuccetelli, Erika Poggiali, Sergio Bernardini, Giovanni Battista Vadacca, Andrea Magnacavallo, Paolo Immovilli, Donata Guidetti, Domenica Zaino, Chiara Terracciano

المصدر: Clinica Chimica Acta; International Journal of Clinical Chemistry
Clinica Chimica Acta

مصطلحات موضوعية: Male, 0301 basic medicine, CoVID-19, Clinical Biochemistry, Respiratory monitoring, Biochemistry, Gastroenterology, chemistry.chemical_compound, Patient Admission, 0302 clinical medicine, Fraction of inspired oxygen, Medicine, Respiratory function, Respiratory system, Aged, 80 and over, biology, General Medicine, Middle Aged, C-Reactive Protein, Italy, 030220 oncology & carcinogenesis, Female, Coronavirus Infections, Respiratory Insufficiency, CRP, Adult, medicine.medical_specialty, LDH, Pneumonia, Viral, Article, Betacoronavirus, Young Adult, 03 medical and health sciences, Predictive Value of Tests, Internal medicine, Lactate dehydrogenase, Humans, Pandemics, Aged, Retrospective Studies, Biochemistry, medical, acute respiratory failure, L-Lactate Dehydrogenase, Clinical Laboratory Techniques, SARS-CoV-2, business.industry, Biochemistry (medical), C-reactive protein, Italian epidemic, 030104 developmental biology, Respiratory failure, chemistry, biology.protein, Creatine kinase, business, Biomarkers, CoVID-19 pneumonia

الوصف: Highlights • Elevated LDH and CRP serum concentrations are associated to respiratory failure in CoVID-19 patients.
Objective The dramatic worldwide CoVID-19 infection requires the identification of a reliable and inexpensive tool to quickly discriminate patients with a more unfavorable outcome. Methods We performed routine laboratory tests suitable to identify tissue damage and inflammatory status in 123 consecutive CoVID-19 patients admitted to the Emergency Department of the hospital of Piacenza (Emilia-Romagna, Northern Italy). The results were correlated with patients’ respiratory function evaluated by the partial pressure of arterial oxygen to fraction of inspired oxygen ratio (PaO2/FiO2). Results: The most common laboratory abnormalities were lymphocytopenia and elevated values of C-reactive protein (CRP) and lactate dehydrogenase (LDH). Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatine kinase (CK) were also increased. The respiratory performance (PaO2/FiO2) showed a strong inverse correlation with LDH (r= 0.62, r2 0.38, p value< 0.0001) and CRP (r= 0.55, r2 0.31, p value< 0.0001). PaO2/FiO2 values also showed a significant inverse correlation with age (r= -0.37, p< 0.0001), AST (r= -0.31, p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::743682d2524dafd43fd8e85497462da5Test
https://doi.org/10.1016/j.cca.2020.06.012Test

المؤلفون: Zhaolei Cui, Yansong Chen, Yan Chen, Yingfeng Lin, Minhua Hu, Shuyu Zhang, Lingying Kong

المصدر: Clinica Chimica Acta. 503:203-209

مصطلحات موضوعية: Adult, Male, 0301 basic medicine, Clinical Biochemistry, Breast Neoplasms, Exosomes, Biochemistry, Exosome, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Breast cancer, Antigen, Recurrence, Lactate dehydrogenase, Biomarkers, Tumor, medicine, Humans, RNA, Messenger, L-Lactate Dehydrogenase, business.industry, Biochemistry (medical), Cancer, General Medicine, Middle Aged, Prognosis, medicine.disease, Survival Analysis, Isoenzymes, Treatment Outcome, 030104 developmental biology, chemistry, Area Under Curve, Case-Control Studies, 030220 oncology & carcinogenesis, Cancer research, Immunohistochemistry, Cancer/testis antigens, Biomarker (medicine), Female, business

الوصف: Lactate dehydrogenase C4 (LDH-C4) as a cancer/testis antigen (CTA) is abnormally expressed in some malignant tumors. However, the expression and clinical significance of LDH-C4 in breast cancer (BC) has not been characterized.We determined LDHC mRNA expression in serum and serum-derived exosomes of BC patients by quantitative RT-PCR. We also evaluated the protein expression of LDH-C4 in BC tissues using high-throughput tissue microarray analysis and immunohistochemistry.Our results showed high mRNA expression level of LDHC in serum and serum-derived exosomes of BC patients. The LDHC level in serum and exosomes could distinguish BC cases from healthy individuals based on their AUCs of 0.9587 and 0.9464, respectively. Besides, the LDHC level in exosomes of BC patients associated with tumor size, and positively correlated with HER2 and Ki-67 expressions (all with P 0.05). Serum and exosomal level of LDHC negatively correlated with medical treatment and positively with the recurrence of BC. Survival analysis showed that LDH-C4 expression negatively correlated with BC prognosis.Serum and exosomal LDHC may be an effective indicator for the diagnosis, efficacy evaluation, and monitoring the recurrence of BC. LDH-C4 may act as a biomarker that predicts BC prognosis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33aee181c2d05bfeef929e7733d01421Test
https://doi.org/10.1016/j.cca.2019.11.032Test

المؤلفون: Jun Zhang, Jiangnan Chen, Yan Xia, Wei Zheng, Xinyou Xie, Shijin Yuan, Xiaoping Xu, Yan Zhang

المصدر: Clinica Chimica Acta
Clinica Chimica Acta; International Journal of Clinical Chemistry

مصطلحات موضوعية: Male, 0301 basic medicine, Time Factors, Clinical Biochemistry, AST, aspartate aminotransferase, SII, systemic immune-inflammation index, Biochemistry, Nomogram, AUC, area under the curve, Leukocyte Count, MERS-CoV, middle east respiratory syndrome coronavirus, COVID-19 Testing, 0302 clinical medicine, WBC, white blood cell count, RBC, red blood cell count, AMC, absolute monocyte count, COVID-19, coronavirus disease 2019, Isolation length, LDH, lactate dehydrogenase, medicine.diagnostic_test, Area under the curve, General Medicine, Middle Aged, PNI, prognostic nutrition index, CT, computed tomography, Hospitalization, Activated partial thromboplastin time, Area Under Curve, 030220 oncology & carcinogenesis, Quarantine, CRP, C-reactive protein, Female, Partial Thromboplastin Time, Partial thromboplastin time, Adult, China, medicine.medical_specialty, Isolation (health care), Coronavirus disease 2019 (COVID-19), RT-PCR, reverse transcription-polymerase chain reaction, SARS-CoV, severe acute respiratory syndrome coronavirus, Physical Distancing, C-index, Concordance index, SARS-CoV-2, severe acute respiratory syndrome coronavirus 2, Antiviral Agents, Article, WHO, World Health Organization, 03 medical and health sciences, Absolute eosinophil count, ALT, alanine aminotransferase, PT, prothrombin time, Internal medicine, medicine, AEC, absolute eosinophil count, Humans, CDC, Centers for Disease Control, APTT, activated partial thromboplastin time, Proportional Hazards Models, Retrospective Studies, Biochemistry, medical, business.industry, Proportional hazards model, Biochemistry (medical), COVID-19, ALC, absolute lymphocyte count, Reproducibility of Results, Retrospective cohort study, Ct, Cycle threshold, Training cohort, Eosinophils, Nomograms, 030104 developmental biology, ANC, absolute neutrophil count, business

الوصف: Highlights • Non-severe COVID-19 patients have abnormal laboratory investigations. • Patients with prolonged pretreatment APTT have a longer isolation length. • Patients with elevated eosinophils after treatment have a shorter isolation length. • A nomogram could help to predict isolation probability at 11-, 16- and 21-day.
Background Majority coronavirus disease 2019 (COVID-19) patients are classified as mild and moderate (non-severe) diseases. We aim to develop a model to predict isolation length for non-severe patients. Methods Among 188 non-severe patients, 96 patients were enrolled as training cohort to identify factors associated with isolation length via Cox regression model and develop a nomogram. Other 92 patients formed as validation cohort to validate nomogram. Concordance index (C-index), area under the curve (AUC) and calibration curves were used to evaluated nomogram. Results Increasing absolute eosinophil count (AEC) after admission was correlated with shorter isolation length (P = 0.02). Baseline activated partial thromboplastin time (APTT) > 30 s was correlated with longer isolation length (P = 0.03). A nomogram to predict isolation probability at 11-, 16- and 21-day was developed and validated. The C-indices of training and validation cohort were 0.604 and 0.682 respectively. Both cohorts showed a good discriminative ability (AUC, 11-day: 0.646 vs 0.730; 16-day: 0.663 vs 0.750; 21-day: 0.711 vs 0.783; respectively) and calibration power. Conclusions Baseline APTT and dynamic change of AEC were two significant factors associated with isolation length of non-severe patients. Nomogram could predict isolation probability for each patient to estimate appropriate quarantine length.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05f7b8c31016bf4dd023e0346dae27f0Test
https://doi.org/10.1016/j.cca.2020.11.019Test

المؤلفون: Katia Stankovic Stojanovic, François Lionnet

المصدر: Clinica Chimica Acta. 458:99-102

مصطلحات موضوعية: Pathology, medicine.medical_specialty, Necrosis, Clinical Biochemistry, Cell, Anemia, Sickle Cell, Hemolysis, Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Lactate dehydrogenase, medicine, Humans, Platelet activation, Pathological, L-Lactate Dehydrogenase, business.industry, Biochemistry (medical), General Medicine, medicine.disease, Pathophysiology, medicine.anatomical_structure, chemistry, 030220 oncology & carcinogenesis, medicine.symptom, business, Vasoconstriction, 030215 immunology

الوصف: Lactate dehydrogenase (LDH) activity is elevated in many pathological states. Interest in LDH activity in sickle cell disease (SCD) has developed out of an increased comprehension of the pathophysiological process and the clinical course of the disease. Elevated LDH activity in SCD comes from various mechanisms, especially intravascular hemolysis, as well as ischemia-reperfusion damage and tissular necrosis. Intravascular hemolysis is associated with vasoconstriction, platelet activation, endothelial damage, and vascular complications. LDH has been used as a diagnostic and prognostic factor of acute and chronic complications. In this review we have evaluated the literature where LDH activity was examined during steady-state or acute conditions in SCD.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d262b9d94ba1687553eb439ca1a5bdd1Test
https://doi.org/10.1016/j.cca.2016.04.035Test

المؤلفون: Irene Blankenburg, Volker Heinemann, Petra Stieber, Dorit Di Gioia, Dorothea Nagel

المصدر: Clinica Chimica Acta. 461:1-7

مصطلحات موضوعية: Adult, 0301 basic medicine, Oncology, medicine.medical_specialty, Receptor, ErbB-2, Clinical Biochemistry, CA 15-3, Breast Neoplasms, Biochemistry, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, Carcinoembryonic antigen, Antigen, Antigens, Neoplasm, Internal medicine, Biomarkers, Tumor, medicine, Humans, CYFRA 21-1, Early Detection of Cancer, Aged, Retrospective Studies, Tumor marker, Keratin-19, L-Lactate Dehydrogenase, biology, business.industry, Mucin-1, Biochemistry (medical), C-reactive protein, General Medicine, Middle Aged, medicine.disease, Metastatic breast cancer, Carcinoembryonic Antigen, C-Reactive Protein, 030104 developmental biology, CA-125 Antigen, 030220 oncology & carcinogenesis, biology.protein, Female, Neoplasm Recurrence, Local, business

الوصف: Background Kinetics of CA 15-3 and CEA have a high specificity in the early detection of metastatic breast cancer (MBC). However, this high specificity is associated with a lack of sensitivity. To decrease the number of false negative patients, the additional diagnostic potential of an extended panel of biomarkers was evaluated. Methods This analysis was performed as part of a large follow-up study (1998–2010) evaluating 813 patients with a median follow-up of 63 months. After primary therapy, all patients underwent tumor marker monitoring for CEA and CA 15-3 at 6-week intervals. A reproducible previously defined increase (≥ 100%) based on the individual baseline value of each patient was considered as a strong indicator of MBC. For the present analysis, we retrospectively evaluated 1011 blood samples from 95 patients. Forty-seven of these had metastatic disease for the first time at the time of this evaluation, while the remaining 48 patients showed no evidence of disease. The sera of these patients were additionally assessed for the following parameters: cancer antigen (CA) 125, cytokeratin-19 soluble fragment (CYFRA 21-1), HER2 shed antigen, lactate-dehydrogenase (LDH) and C-reactive protein (CRP). Results 26 of 47 patients with MBC showed a reproducible tumor marker increase of at least CEA and/or CA 15-3 (55.3%, true-positive). The remaining 21 patients with MBC showed no increase in CEA or CA 15-3 (44.7%, false negative, FN). By combining all markers mentioned above, 41 of 47 patients with MBC showed a reproducible marker increase with a sensitivity of 87.2% and specificity of 100%. Conclusion This retrospective analysis indicates that a panel of biomarkers can increase the sensitivity of the CA 15-3/CEA combination without loss of specificity. The combined use is therefore helpful for early detection of MBC.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b06fb3147694a04ac256266fd5953accTest
https://doi.org/10.1016/j.cca.2016.07.014Test

المؤلفون: Sara Fernández-Landázuri, Estibaliz Alegre, Alvaro Gonzalez, Carmen P. Rodriguez, Salvador Martín-Algarra, José I. Echeveste, Jose Luis Perez Gracia, Miguel F. Sanmamed, Maria D. Lozano, Omar Esteban Carranza, Leyre Zubiri

المصدر: Clinica Chimica Acta. 429:168-174

مصطلحات موضوعية: Male, Proto-Oncogene Proteins B-raf, Oncology, medicine.medical_specialty, Pathology, Skin Neoplasms, endocrine system diseases, Clinical Biochemistry, Biochemistry, Internal medicine, Biomarkers, Tumor, medicine, Humans, Neoplasm Metastasis, Vemurafenib, Prospective cohort study, Melanoma, Protein Kinase Inhibitors, neoplasms, Tumor marker, Extracellular Matrix Proteins, L-Lactate Dehydrogenase, business.industry, S100 Proteins, Biochemistry (medical), Melanoma inhibitory activity, Dabrafenib, General Medicine, Middle Aged, Prognosis, medicine.disease, digestive system diseases, Neoplasm Proteins, Clinical trial, Treatment Outcome, Expanded access, Female, business, medicine.drug

الوصف: BRAF V600 mutation has been reported in more than 50% of melanoma cases and its presence predicts clinical activity of BRAF inhibitors (iBRAF). We evaluated the role of MIA, S100 and LDH to monitor iBRAF efficiency in advanced melanoma patients presenting BRAF V600 mutations. This was a prospective study of melanoma patients harboring the BRAF V600 mutation and treated with iBRAF within a clinical trial (dabrafenib) or as part of an expanded access program (vemurafenib). MIA, S100 and LDH were analyzed in serum at baseline, and every 4–6 weeks during treatment. Eighteen patients with melanoma stages IIIc–IV were enrolled with 88.8% of response rate to iBRAF. Baseline concentrations of all the tumor markers correlated with tumor burden. MIA and S100 concentrations decreased significantly one month after the beginning of treatment and, upon progression, their concentrations increased significantly above the minimum levels previously achieved. MIA levels lower than 9 μg/L one month after the beginning of treatment and S100 concentrations lower than 0.1 μg/L at the moment of best response were associated with improved progression-free survival. In conclusion, MIA and S100 are useful to monitor response in melanoma patients treated with iBRAF.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbe1cc23ccd3014a765df92fd9ea708cTest
https://doi.org/10.1016/j.cca.2013.11.034Test

المؤلفون: Pachiappan Chitra, Balakrishnan Bakthavatsalam, Thayumanavan Palvannan

المصدر: Clinica Chimica Acta. 412:1151-1154

مصطلحات موضوعية: CD8 Antigens, Clinical Biochemistry, CD4-CD8 Ratio, HIV Infections, Biochemistry, Leukocyte Count, Immune system, Acquired immunodeficiency syndrome (AIDS), Immunity, Antiretroviral Therapy, Highly Active, Humans, Medicine, Stage (cooking), L-Lactate Dehydrogenase, medicine.diagnostic_test, Beta-2 microglobulin, business.industry, Biochemistry (medical), virus diseases, General Medicine, medicine.disease, Real-time polymerase chain reaction, Immunoassay, Immunology, Disease Progression, RNA, Viral, beta 2-Microglobulin, business, Biomarkers, CD8

الوصف: Background Acquired immune deficiency syndrome (AIDS) defines the end stage of Human immunodeficiency viral (HIV) infection before the introduction of highly active antiretroviral therapy (HAART). This study was carried out to assess the serum β-2 microglobulin (B2M) as a marker for progression of HIV infected patients undergoing HAART. Methods Blood samples were collected from 50 subjects of HIV infected patients residing at semi urban area undergoing treatment at Chellam Hospital, Salem, India. Twenty five age-matched healthy subjects were taken as control group. Serum B2M level was measured by using enzyme immunoassay, absolute CD4 and CD8 counts were carried out by single platform (SP) technology using a flow cytometer. Viral RNA was measured by real time PCR. The serum LDH level and total WBC count were also measured. Results Our result showed a statistically high significant increase in B2M in HIV patients on HAART non complaint group whereas absolute CD4, CD8 count, CD4/CD8 ratio and WBC count were decreased significantly when compared to control and HAART complaint group. Statistically a significant negative correlation was observed between B2M and absolute CD4, CD8 count, CD4/CD8 ratio and WBC count. B2M showed a significantly positive correlation with viral RNA and LDH values. Conclusions The increase of B2M and reduced absolute CD4, CD8 count, CD4/CD8 ratio and WBC count in HIV patients on HAART non complaint group may have a contributory role in the immune progression of HIV with interruption of HAART. B2M plays an important role in the diagnosis of HIV and might indicate HIV progression.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1eb4c53b75b958a4995d98d3de336305Test
https://doi.org/10.1016/j.cca.2011.01.037Test