المؤلفون: Elena Aloisio, Giulia Colombo, Alberto Dolci, Mauro Panteghini, Claudia Arrigo

المصدر: Clinica Chimica Acta; International Journal of Clinical Chemistry

مصطلحات موضوعية: Clinical Biochemistry, AST, aspartate aminotransferase, Liver injury, Systemic inflammation, Biochemistry, Gastroenterology, law.invention, chemistry.chemical_compound, fluids and secretions, law, polycyclic compounds, Medicine, IL-6, interleukin-6, LDH, lactate dehydrogenase, biology, Liver Diseases, virus diseases, Alanine Transaminase, R2, coefficient of determination, General Medicine, ICU, intensive care unit, Intensive care unit, P-5′-P, pyridoxal-5-phosphate, CRP, C-reactive protein, Alkaline phosphatase, medicine.symptom, medicine.medical_specialty, Aspartate aminotransferase, digestive system, Article, SARS-CoV-2, severe acute respiratory syndrome coronavirus 2 COVID-19. 2019 coronavirus disease, ALT, alanine aminotransferase, Lactate dehydrogenase, Internal medicine, Humans, Clinical significance, IFCC, International Federation of Clinical Chemistry and Laboratory Medicine, Aspartate Aminotransferases, IQR, interquartile range, ALP, alkaline phosphatase, SARS-CoV-2, business.industry, Biochemistry (medical), URL, upper reference limit, COVID-19, medicine.disease, digestive system diseases, OR, odds ratio, CI, confidence interval, HI, hemolysis index, GGT, γ-glutamyltransferase, R, correlation coefficient, Alanine transaminase, chemistry, biology.protein, Creatine kinase, business, CK, creatine kinase

الوصف: Background Aspartate aminotransferase (AST) is often increased in COVID-19 and, in some studies, AST abnormalities were associated with mortality risk. Methods 2054 hospitalized COVID-19 patients were studied. To identify sources of AST release, correlations between AST peak values and other biomarkers of tissue damage, i.e., alanine aminotransferase (ALT) for hepatocellular damage, creatine kinase (CK) for muscle damage, lactate dehydrogenase for multiorgan involvement, alkaline phosphatase and γ-glutamyltransferase for cholestatic injury, and C-reactive protein (CRP) for systemic inflammation, were performed and coefficients of determination estimated. The role of AST to predict death and intensive care unit admission during hospitalization was also evaluated. All measurements were performed using standardized assays. Results AST was increased in 69% of patients. Increases could be fully explained by summing the effects of hepatocellular injury [AST dependence from ALT, 66.8% [95% confidence interval (CI): 64.5–69.1)] and muscle damage [AST dependence from CK, 42.6% (CI: 39.3–45.8)]. We were unable to demonstrate an independent association of AST increases with worse outcomes. Conclusion The mechanisms for abnormal AST in COVID-19 are likely multifactorial and a status related to tissue suffering could play a significant role. The clinical significance of AST elevations remains unclear.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3d1d325846e5aacf52eb8f0ef551a94Test
https://doi.org/10.1016/j.cca.2021.08.012Test

المؤلفون: Guojun Zhang, Maidinaimu Aibibula, Ning Feng, Huijun Li, Hui Zhao, Jie Lv, Bin Li, Fengming Tian, Xiumin Ma, Liang Wang

المصدر: Clinica Chimica Acta. 510:665-670

مصطلحات موضوعية: 0301 basic medicine, medicine.medical_specialty, Clinical Biochemistry, Disease, Biochemistry, Gastroenterology, Procalcitonin, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, Lactate dehydrogenase, medicine, Humans, In patient, Interleukin 6, L-Lactate Dehydrogenase, biology, Interleukin-6, business.industry, Biochemistry (medical), C-reactive protein, General Medicine, medicine.disease, Systemic inflammatory response syndrome, C-Reactive Protein, 030104 developmental biology, Pancreatitis, chemistry, 030220 oncology & carcinogenesis, Acute Disease, biology.protein, Acute pancreatitis, business, Biomarkers

الوصف: Acute pancreatitis (AP) is an inflammatory disease with rapid progression. In severe cases, it can cause systemic inflammatory response syndrome (SIRS), multiple organ failure (POF) and even death. The study aimed to investigate the diagnostic value of C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6) and lactate dehydrogenase (LDH) in patients with severe AP.AP patients (n = 153) divided into mild AP patients (n = 81) and severe AP patients (n = 72) were selected from June 2014 to June 2016. The demographic information (age, sex) and the hematological parameters (WBC, PLT, CRP, PCT, IL-6, LDH and so on) were analyzed.Significant differences were found out of CRP, PCT, IL-6 and LDH values between AP patients and controls (P 0.05), even those results had significant difference between MAP group and SAP group (P 0.05). In SAP group, the cut-off values of CRP, PCT, IL-6 and LDH were 16.62, 2.29, 16.66, 273.04; sensitivity 55.6%, 77.8%, 80.2%, 82.7%; specificity 73%, 94%, 85%, 96% and AUC 0.637, 0.929, 0.886, 0.919, respectively. The AUC of combined detection of CRP, PCT, IL-6 and LDH was 0.989 (95%CI).The combined detection of CRP, PCT, IL-6 and LDH has a high diagnostic value for judging the severity of AP.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50ccc81012d8efe6161e39ac657aa3cbTest
https://doi.org/10.1016/j.cca.2020.08.029Test

المؤلفون: Giulia Losi, Alessandro Dacrema, Luca Rovero, Andrea Vercelli, Marzia Nuccetelli, Erika Poggiali, Sergio Bernardini, Giovanni Battista Vadacca, Andrea Magnacavallo, Paolo Immovilli, Donata Guidetti, Domenica Zaino, Chiara Terracciano

المصدر: Clinica Chimica Acta; International Journal of Clinical Chemistry
Clinica Chimica Acta

مصطلحات موضوعية: Male, 0301 basic medicine, CoVID-19, Clinical Biochemistry, Respiratory monitoring, Biochemistry, Gastroenterology, chemistry.chemical_compound, Patient Admission, 0302 clinical medicine, Fraction of inspired oxygen, Medicine, Respiratory function, Respiratory system, Aged, 80 and over, biology, General Medicine, Middle Aged, C-Reactive Protein, Italy, 030220 oncology & carcinogenesis, Female, Coronavirus Infections, Respiratory Insufficiency, CRP, Adult, medicine.medical_specialty, LDH, Pneumonia, Viral, Article, Betacoronavirus, Young Adult, 03 medical and health sciences, Predictive Value of Tests, Internal medicine, Lactate dehydrogenase, Humans, Pandemics, Aged, Retrospective Studies, Biochemistry, medical, acute respiratory failure, L-Lactate Dehydrogenase, Clinical Laboratory Techniques, SARS-CoV-2, business.industry, Biochemistry (medical), C-reactive protein, Italian epidemic, 030104 developmental biology, Respiratory failure, chemistry, biology.protein, Creatine kinase, business, Biomarkers, CoVID-19 pneumonia

الوصف: Highlights • Elevated LDH and CRP serum concentrations are associated to respiratory failure in CoVID-19 patients.
Objective The dramatic worldwide CoVID-19 infection requires the identification of a reliable and inexpensive tool to quickly discriminate patients with a more unfavorable outcome. Methods We performed routine laboratory tests suitable to identify tissue damage and inflammatory status in 123 consecutive CoVID-19 patients admitted to the Emergency Department of the hospital of Piacenza (Emilia-Romagna, Northern Italy). The results were correlated with patients’ respiratory function evaluated by the partial pressure of arterial oxygen to fraction of inspired oxygen ratio (PaO2/FiO2). Results: The most common laboratory abnormalities were lymphocytopenia and elevated values of C-reactive protein (CRP) and lactate dehydrogenase (LDH). Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatine kinase (CK) were also increased. The respiratory performance (PaO2/FiO2) showed a strong inverse correlation with LDH (r= 0.62, r2 0.38, p value< 0.0001) and CRP (r= 0.55, r2 0.31, p value< 0.0001). PaO2/FiO2 values also showed a significant inverse correlation with age (r= -0.37, p< 0.0001), AST (r= -0.31, p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::743682d2524dafd43fd8e85497462da5Test
https://doi.org/10.1016/j.cca.2020.06.012Test

المؤلفون: Mani Arulkumar, Palanivel Sathishkumar, Sakayanathan Penislusshiyan, Jayaraman Angayarkanni, Raji Vijayan, Thayumanavan Palvannan

المصدر: Clinica Chimica Acta. 471:206-215

مصطلحات موضوعية: Adult, Male, 0301 basic medicine, Clinical Biochemistry, Population, India, 030204 cardiovascular system & hematology, Biochemistry, Arylesterase, Fluorides, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Fluoride toxicity, Lactate dehydrogenase, Humans, education, education.field_of_study, biology, Aryldialkylphosphatase, Chemistry, Biochemistry (medical), Paraoxonase, General Medicine, 030104 developmental biology, Delta-aminolevulinic acid dehydratase, biology.protein, Female, Triphosphatase, Carboxylic Ester Hydrolases, Fluoride, Biomarkers, Water Pollutants, Chemical, Environmental Monitoring

الوصف: Background Toxicity due to excess fluoride concentration in drinking water is of great concern in people who rely only on the ground water as their water source in many region of the world. Methods We collected samples and examined the toxicity of fluoride in a population residing at Salem, Dharmapuri and Krishnagiri districts of Tamil Nadu, India and measured HDL bound enzyme (PON1), erythrocyte membrane bound enzymes (acetylcholinesterase, AChE) and adenosine 5′ triphosphatase (ATPases), plasma enzyme (butyrylcholinesterase, BChE) and rate limiting enzyme in heme biosynthesis (delta aminolevulinic acid dehydratase, δ-ALAD) activities. Results In fluorosis patients, formation of lipid peroxidation product was more in erythrocytes than in plasma. The observation further revealed that there was 50% reduction in the activity of HDL bound anti atherogenic enzyme-paraoxonase (PON1). The activities of membrane bound and signaling enzymes (acetylcholinesterase - AChE and adenosine 5′ triphosphatase - ATPase) of erythrocyte were also diminished. These results suggested that there was defectiveness in the signaling and energy metabolism in fluorosis patients. Altered isoenzyme pattern of lactate dehydrogenase (LDH) in fluorosis samples was observed. Furthermore, the result suggested that both the heart (LDH 1) and liver (LDH 5) were most affected by fluoride toxicity. The study also provided reference values for tests which are used to predict the severity of fluoride toxicity. Conclusion The toxic effect of fluoride was due to the collective effects on vital protective system rather than single factor.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eebf7490e66c7adbd1b1e5d8a453ed0bTest
https://doi.org/10.1016/j.cca.2017.05.036Test

المؤلفون: Irene Blankenburg, Volker Heinemann, Petra Stieber, Dorit Di Gioia, Dorothea Nagel

المصدر: Clinica Chimica Acta. 461:1-7

مصطلحات موضوعية: Adult, 0301 basic medicine, Oncology, medicine.medical_specialty, Receptor, ErbB-2, Clinical Biochemistry, CA 15-3, Breast Neoplasms, Biochemistry, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, Carcinoembryonic antigen, Antigen, Antigens, Neoplasm, Internal medicine, Biomarkers, Tumor, medicine, Humans, CYFRA 21-1, Early Detection of Cancer, Aged, Retrospective Studies, Tumor marker, Keratin-19, L-Lactate Dehydrogenase, biology, business.industry, Mucin-1, Biochemistry (medical), C-reactive protein, General Medicine, Middle Aged, medicine.disease, Metastatic breast cancer, Carcinoembryonic Antigen, C-Reactive Protein, 030104 developmental biology, CA-125 Antigen, 030220 oncology & carcinogenesis, biology.protein, Female, Neoplasm Recurrence, Local, business

الوصف: Background Kinetics of CA 15-3 and CEA have a high specificity in the early detection of metastatic breast cancer (MBC). However, this high specificity is associated with a lack of sensitivity. To decrease the number of false negative patients, the additional diagnostic potential of an extended panel of biomarkers was evaluated. Methods This analysis was performed as part of a large follow-up study (1998–2010) evaluating 813 patients with a median follow-up of 63 months. After primary therapy, all patients underwent tumor marker monitoring for CEA and CA 15-3 at 6-week intervals. A reproducible previously defined increase (≥ 100%) based on the individual baseline value of each patient was considered as a strong indicator of MBC. For the present analysis, we retrospectively evaluated 1011 blood samples from 95 patients. Forty-seven of these had metastatic disease for the first time at the time of this evaluation, while the remaining 48 patients showed no evidence of disease. The sera of these patients were additionally assessed for the following parameters: cancer antigen (CA) 125, cytokeratin-19 soluble fragment (CYFRA 21-1), HER2 shed antigen, lactate-dehydrogenase (LDH) and C-reactive protein (CRP). Results 26 of 47 patients with MBC showed a reproducible tumor marker increase of at least CEA and/or CA 15-3 (55.3%, true-positive). The remaining 21 patients with MBC showed no increase in CEA or CA 15-3 (44.7%, false negative, FN). By combining all markers mentioned above, 41 of 47 patients with MBC showed a reproducible marker increase with a sensitivity of 87.2% and specificity of 100%. Conclusion This retrospective analysis indicates that a panel of biomarkers can increase the sensitivity of the CA 15-3/CEA combination without loss of specificity. The combined use is therefore helpful for early detection of MBC.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b06fb3147694a04ac256266fd5953accTest
https://doi.org/10.1016/j.cca.2016.07.014Test

المؤلفون: Yoshihisa Shimizu, Kiyoshi Ichihara

المصدر: Clinica Chimica Acta. 446:64-72

مصطلحات موضوعية: Adult, Electrophoresis, Male, Clinical Biochemistry, Physiology, Biochemistry, Isozyme, Young Adult, Sex Factors, Asian People, Reference Values, ABO blood group system, Humans, Derivation, Amylase, Autoanalysis, L-Lactate Dehydrogenase, biology, Biochemistry (medical), Age Factors, Reproducibility of Results, General Medicine, Middle Aged, Alkaline Phosphatase, Reference intervals, Isoenzymes, Reference values, Amylases, Multivariate Analysis, biology.protein, Regression Analysis, Female, Analysis of variance, Body mass index

الوصف: Background Sources of variation (SV) of ALP, LDH, and amylase isozymes were explored. Methods We analyzed 3511 sera from well-defined healthy individuals recruited during the 2009 Asian project for derivation of common reference intervals (RIs). Up-to-date electrophoresis auto-analyzer and reagents were employed for high resolution and reproducibility. SVs including sex, age, body mass index (BMI), ABO blood groups, and levels of drinking, smoking, and exercise were analyzed by multiple regression analysis. RIs were determined by parametric methods after refining healthy individuals by use of latent reference values exclusion method. Results Age-related changes in ALP2–3 were different in females: ALP2, linear increase from 20–64 y; ALP3, lowering until 45 y and rising steeply thereafter. ALP2 increased with BMI especially in females. ALP5 was barely detectable except in blood-types O and B. Age-related increases in LDH1–LDH3 were noted in females, whereas BMI-related increases were found only for LDH2–LDH5 in both sexes. Pancreatic amylase showed age-related increase in females and was slightly higher in blood-type O. RIs for absolute and relative activities of each isozyme were derived in consideration of sex and age. Conclusions Investigation of these isozymes revealed various age-, BMI-, and blood-type-related changes that are all relevant in clinical interpretation of enzyme test results.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64d914f914afd90e6dc70000ff950678Test
https://doi.org/10.1016/j.cca.2015.03.034Test

المؤلفون: Evelien Bauwens, Veronique Stove, Tom Van Maerken, Matthijs Oyaert, Joris R. Delanghe

المصدر: Clinica Chimica Acta. 468:209-210

مصطلحات موضوعية: medicine.medical_specialty, biology, Catalytic concentration, Biochemistry (medical), Clinical Biochemistry, L-Lactate dehydrogenase, General Medicine, Biochemistry, chemistry.chemical_compound, Endocrinology, chemistry, Lactate dehydrogenase, Internal medicine, biology.protein, medicine, Neuraminidase

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::6d63be5b7027f973eac39077927453a9Test
https://doi.org/10.1016/j.cca.2017.03.011Test

المؤلفون: Palaninathan Varalakshmi, Perinkulam Ravi Deepa

المصدر: Clinica Chimica Acta. 339:105-115

مصطلحات موضوعية: Male, medicine.medical_specialty, Albinism, Arteriosclerosis, Clinical Biochemistry, Serum albumin, Certoparin sodium, Kidney, Biochemistry, chemistry.chemical_compound, Lactate dehydrogenase, Internal medicine, medicine, Animals, Urea, Rats, Wistar, Creatine Kinase, Serum Albumin, Transaminases, Creatinine, L-Lactate Dehydrogenase, Triglyceride, biology, Cholesterol, Biochemistry (medical), Heart, General Medicine, Heparin, Heparin, Low-Molecular-Weight, Alkaline Phosphatase, Creatine, Rats, Uric Acid, Endocrinology, Liver, chemistry, biology.protein, Diet, Atherogenic, Uric acid, medicine.drug

الوصف: Background : The association of atherosclerosis and hypercholesterolemia is well known. Hypercholesterolemic diet-induced atherogenesis is a widely accepted experimental model that is amenable to exploration of both the disease as well as therapeutic interventions. We evaluated the role of low molecular weight heparin (LMWH) in modulating the early biochemical changes in atherogenesis. Methods : Male Wistar rats (140±10 g) were fed an atherogenic diet comprising of normal rat chow supplemented with 4% cholesterol, 1% cholic acid and 0.5% thiouracil (CCT diet) for 2 weeks. While one of the CCT diet-fed group served as the untreated pathologic model, the other group received LMWH (Certoparin sodium, Troparin ® ; 300 μg/day/rat s.c.) treatment, commencing on day 8 and continued for 1 week. Results : Decreased concentrations of serum albumin and increased serum urea, uric acid and creatinine concentrations were normalized by LMWH treatment. The atherogenic diet induced abnormal rise in the activities of lactate dehydrogenase, aminotransferases and alkaline phosphatase, as well as the high serum cholesterol and triglyceride concentrations were restored to near control values in the treated group. LMWH administration prevented the hypertrophic cardiac histology and fatty changes in the liver in early atherogenesis. Conclusion : The present study encapsulates the early cellular abnormalities in the heart, liver and kidney tissues of atherogenic diet fed rats. Treatment with LMWH affords considerable protection to the tissues challenged by hypercholesterolemia, evidenced by its correction of lipemia and restoration of serum and tissue indices of injury, to normalcy. LMWH intervention minimized the atherogenic diet-induced histopathological lesions in heart, liver and kidney tissues.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f26d4b814c169d20a95fb164ace7287Test
https://doi.org/10.1016/j.cccn.2003.09.021Test

المؤلفون: Saeed A. Jortani, Roland Valdes

المصدر: Clinica Chimica Acta. 284:135-140

مصطلحات موضوعية: medicine.medical_specialty, Cardiac troponin, Clinical Biochemistry, Biochemistry, Proficiency testing, Humans, Medicine, Intensive care medicine, Creatine Kinase, L-Lactate Dehydrogenase, biology, business.industry, Biochemistry (medical), General Medicine, Troponin, Coronary heart disease, Lactate dehydrogenase isoenzymes, Surgery, Isoenzymes, Clinical Practice, biology.protein, Creatine kinase, Myocardial disease, Cardiomyopathies, business, Biomarkers

الوصف: Publications on the development and use of myocardial markers have exploded in the decade of the 1990s. According to subscriptions to proficiency testing surveys, enzymatic measurement of creatine kinase and lactate dehydrogenase isoenzymes have largely been replaced by CK-MB mass immunoassays. Laboratories reporting use of myoglobin and cardiac troponin have increased tremendously over the past few years. In this field of medicine where there have been dramatic changes, development of consensus guidelines can be helpful to provide assistance to clinicians and laboratorians as to how they can make the best use of new cardiac markers for clinical practice.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0c3f3dc496a4b8e9c60b9a1d0e8f1daTest
https://doi.org/10.1016/s0009-8981Test(99)00075-3

المؤلفون: Seiichiro Ozono, Junichi Kawakami, Atsushi Otsuka, Yoshiyuki Kagawa, Takafumi Naito, Yasuaki Mino

المصدر: Clinica Chimica Acta. 409:56-61

مصطلحات موضوعية: Adult, Male, Erythrocytes, Clinical Biochemistry, Serum albumin, Pharmacology, Mycophenolate, Biochemistry, Mycophenolic acid, chemistry.chemical_compound, Glucuronides, IMP Dehydrogenase, IMP dehydrogenase, Lactate dehydrogenase, medicine, Humans, Inosine-5′-monophosphate dehydrogenase, Kidney transplantation, Creatinine, biology, Chemistry, Biochemistry (medical), General Medicine, Middle Aged, Mycophenolic Acid, medicine.disease, Kidney Transplantation, biology.protein, Female, Immunosuppressive Agents, medicine.drug

الوصف: Background Inosine 5′-monophosphate dehydrogenase (IMPDH) is a target of the immunosuppressant mycophenolic acid (MPA) which is used for the prevention of acute rejection. We evaluated the concentration-dependent effects of MPA and its phenolic (MPAG) and acyl (AcMPAG) glucuronides on IMPDH activity in erythrocytes in an initial and a stable phase. Methods Eight kidney transplant recipients treated with mycophenolate mofetil (MMF) in an initial phase and 104 recipients [56 received MMF (MMF+) and 48 did not (MMF−)] in a stable phase were enrolled. Results IMPDH activity in erythrocytes was inhibited at the peak plasma concentration of MPA in initial kidney transplant recipients. However, median IMPDH activity in erythrocytes was 1.73 times higher in MMF+ than in MMF− kidney transplant recipients (38.7 to 22.4 nmol/g hemoglobin/h, P 12 ) of MPA, MPAG, and AcMPAG in stable kidney transplant recipients were 2.86, 49.9, and 0.256 μg/ml, respectively. In a multivariate analysis, AcMPAG C 12 , MPAG C 12 , MPA C 12 , serum creatinine, age, lactate dehydrogenase, and serum albumin were significant predictors, accounting for interindividual variability of IMPDH ( R 2 = 0.537, P = 0.02). Conclusion IMPDH activity in erythrocytes may be useful indicators of short-term immunosuppression and long-term exposure of MPA.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2471292a9529b0b12c647a09819a10e9Test
https://doi.org/10.1016/j.cca.2009.08.016Test