Transgenic Modeling of a Cardiac Troponin I Mutation Linked to Familial Hypertrophic Cardiomyopathy
| العنوان: | Transgenic Modeling of a Cardiac Troponin I Mutation Linked to Familial Hypertrophic Cardiomyopathy |
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| المؤلفون: | Yan Zhang, Timothy E. Hewett, Atsushi Sanbe, Jeffrey Robbins, Jeanne James, Raisa Klevitsky, Hanna Osinska |
| المصدر: | Circulation Research. 87:805-811 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Male, Genetically modified mouse, medicine.medical_specialty, Physiology, Transgene, Mice, Transgenic, macromolecular substances, Myosins, medicine.disease_cause, Troponin C, Mice, Structure-Activity Relationship, Internal medicine, Troponin I, medicine, Animals, Protein Isoforms, Actin, Mutation, biology, Myocardium, Age Factors, Hypertrophic cardiomyopathy, Cardiomyopathy, Hypertrophic, medicine.disease, Survival Analysis, Troponin, Actins, Phenotype, Endocrinology, Amino Acid Substitution, Models, Animal, cardiovascular system, biology.protein, RNA, Calcium, Female, Cardiology and Cardiovascular Medicine |
| الوصف: | Abstract —Multiple mutations in cardiac troponin I (cTnI) have been associated with familial hypertrophic cardiomyopathy. Two mutations are located in the cTnI inhibitory domain, a highly negatively charged region that alternately binds to either actin or troponin C, depending on the intracellular concentration of calcium. This region is critical to the inhibition of actin-myosin crossbridge formation when intracellular calcium is low. We modeled one of the inhibitory domain mutations, arginine145→glycine (TnI 146Gly in the mouse sequence), by cardiac-specific expression of the mutated protein in transgenic mice. Multiple lines were generated with varying degrees of expression to establish a dose relationship; the severity of phenotype could be correlated directly with transgene expression levels. Transgenic mice overexpressing wild-type cTnI were generated as controls and analyzed in parallel with the TnI 146Gly animals. The control mice showed no abnormalities, indicating that the phenotype of TnI 146Gly was not simply an artifact of transgenesis. In contrast, TnI 146Gly mice showed cardiomyocyte disarray and interstitial fibrosis and suffered premature death. The functional alterations that seem to be responsible for the development of cardiac disease include increased skinned fiber sensitivity to calcium and, at the whole organ level, hypercontractility with diastolic dysfunction. Severely affected lines develop a pathology similar to human familial hypertrophic cardiomyopathy but within a dramatically shortened time frame. These data establish the causality of this mutation for cardiac disease, provide an animal model for understanding the resultant pathogenic structure-function relationships, and highlight the differences in phenotype severity of the troponin mutations between human and mouse hearts. |
| تدمد: | 1524-4571 0009-7330 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35c7ba55f459a389edc1481b41715189Test https://doi.org/10.1161/01.res.87.9.805Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....35c7ba55f459a389edc1481b41715189 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15244571 00097330 |
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