On-Fiber Derivatization of SPME Extracts of Phenol, Hydroquinone and Catechol with GC-MS Detection
| العنوان: | On-Fiber Derivatization of SPME Extracts of Phenol, Hydroquinone and Catechol with GC-MS Detection |
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| المؤلفون: | Ernani Pinto, Ana Maria da Costa Ferreira, Mauricio Yonamine, Emerson Luiz Botelho Lourenço, Sandra Helena Poliselli Farsky |
| المصدر: | Chromatographia. 63:175-179 |
| بيانات النشر: | Springer Science and Business Media LLC, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Detection limit, Chromatography, Hydroquinone, Organic Chemistry, Clinical Biochemistry, Solid-phase microextraction, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, chemistry, Phenol, Selected ion monitoring, Gas chromatography, Gas chromatography–mass spectrometry, Derivatization |
| الوصف: | A gas chromatography-mass spectrometry (GC-MS) method for the simultaneous determination of phenol (PHE), hydroquinone (HQ) and catechol (CAT) in urine was developed and validated. The method was based on the acidic hydrolysis of conjugated phenolic compounds and further extraction of analytes using solid-phase microextraction (SPME). Analytes were extracted by submersing the polar polyacrylate coated fiber (85 µm) into urine (adjusted to pH 3.0 with glacial acetic acid) for 20 min with magnetic stirring. The extracted compounds on the fiber were exposed to hexamethyldisilazane reagent in the vapor phase for 20 min to yield the corresponding trimethysililylated derivates. This on-fiber derivatization procedure allowed the formation of more amenable compounds for GC analysis, without adversely affecting the lifetime of the fiber. The MS was operated in the selected ion monitoring mode (SIM). The limits of detection were 0.3 µg mL−1 for PHE, 0.15 µg mL−1 for HQ and 0.02 µg mL−1 for CAT. Inter and intra-assay precisions were also verified (coefficient of variation < 8%) with the use of deuterated internal standards. This method of GC-MS analysis can be readily utilized to monitor PHE and its metabolites (HQ and CAT) in urine samples. |
| تدمد: | 1612-1112 0009-5893 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::d26763f9b1f985b82bb4e94ed13299ddTest https://doi.org/10.1365/s10337-006-0719-8Test |
| رقم الانضمام: | edsair.doi...........d26763f9b1f985b82bb4e94ed13299dd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16121112 00095893 |
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