A Recombinant Courtship Pheromone Affects Sexual Receptivity in a Plethodontid Salamander
| العنوان: | A Recombinant Courtship Pheromone Affects Sexual Receptivity in a Plethodontid Salamander |
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| المؤلفون: | Lynne D. Houck, Karen M. Kiemnec, Kathleen E. Bowen, Pamela W. Feldhoff, Richard C. Feldhoff, Richard A. Watts, Hilary A. Godwin, Stevan J. Arnold |
| المصدر: | Chemical Senses. 33:623-631 |
| بيانات النشر: | Oxford University Press (OUP), 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Male, Physiology, media_common.quotation_subject, Receptivity, Urodela, Courtship, Sexual Behavior, Animal, Behavioral Neuroscience, Physiology (medical), biology.animal, Animals, Sex Attractants, Mating, media_common, biology, Courtship display, Ecology, biology.organism_classification, Recombinant Proteins, digestive system diseases, Sensory Systems, Cell biology, Sex pheromone, Salamander, Pheromone, Female, Plethodon shermani |
| الوصف: | Pheromones are important chemical signals for many vertebrates, particularly during reproductive interactions. In the terrestrial salamander Plethodon shermani, a male delivers proteinaceous pheromones to the female as part of their ritualistic courtship behavior. These pheromones increase the female's receptivity to mating, as shown by a reduction in courtship duration. One pheromone component in particular is plethodontid receptivity factor (PRF), a 22-kDa protein with multiple isoforms. This protein alone can act as a courtship pheromone that causes the female to be more receptive. We used a bacterial expression system to synthesize a single recombinant isoform of PRF. The recombinant protein was identical to the native PRF, based on mass spectrometry, circular dichroism spectra, and a behavioral bioassay that tested the effects of recombinant PRF (rPRF) on female receptivity (21% reduction in courtship duration). The rPRF appears to mimic the activity of a mixture of PRF isoforms, as well as a mixture of multiple different proteins that comprise the male courtship gland extract. Pheromones that are peptides have been characterized for some vertebrates; to date, however, rPRF is one of only 2 synthesized vertebrate proteins to retain full biological activity. |
| تدمد: | 1464-3553 0379-864X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9967b5ebc114547ce1b32fefcbf2fa78Test https://doi.org/10.1093/chemse/bjn027Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9967b5ebc114547ce1b32fefcbf2fa78 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14643553 0379864X |
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