Water-soluble allyl sulfones for dual site-specific labelling of proteins and cyclic peptides
| العنوان: | Water-soluble allyl sulfones for dual site-specific labelling of proteins and cyclic peptides |
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| المؤلفون: | Seah Ling Kuan, Markus Lamla, Yuzhou Wu, Holger Barth, Sebastian Wiese, Markus Otto, Tao Wang, Patrick Oeckl, Tanja Weil, Andreas Riegger, Stephan Fischer |
| المصدر: | Chemical Science |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | chemistry.chemical_classification, 010405 organic chemistry, Stereochemistry, Reaction step, organic chemicals, food and beverages, Peptide, General Chemistry, Chromophore, 010402 general chemistry, 01 natural sciences, Cyclic peptide, 0104 chemical sciences, Green fluorescent protein, chemistry.chemical_compound, Chemistry, chemistry, Thiol, Lysozyme, Conjugate |
| الوصف: | Allyl sulfones as efficient disulfide rebridging agents for site-specific protein modifications with up to two additional functionalities in water. Water-soluble allyl sulfones provide convenient site-specific disulfide rebridging of native proteins and cyclic peptides. The site-selective functionalization of (a) the peptide hormone somatostatin, (b) the interchain disulfide of bovine insulin and (c) functionalization of the proteins GFP and lysozyme with allyl sulfones proceeds in aqueous solution. Allyl sulfones offer three functionalizable sites that react with thiol containing molecules in a step-wise fashion. Dual labeling of proteins and cyclic peptides is achieved i.e. the attachment of a chromophore and an affinity tag in a single reaction step, which is of great significance for the construction of precise multifunctional peptide and protein conjugates. |
| وصف الملف: | application/pdf |
| تدمد: | 2041-6520 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad02927a87ab83691a0b4a2390f36201Test https://pubmed.ncbi.nlm.nih.gov/29997815Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ad02927a87ab83691a0b4a2390f36201 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20416520 |
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