Cross-conjugation of DNA, proteins and peptides via a pH switch
| العنوان: | Cross-conjugation of DNA, proteins and peptides via a pH switch |
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| المؤلفون: | Oliver Dumele, Klaus Müllen, Seah Ling Kuan, Markus Lamla, Yuzhou Wu, Tao Wang, Tanja Weil, Anne Pfisterer |
| المصدر: | Chemical Science. 4:1889 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Steric effects, chemistry.chemical_compound, Bioconjugation, chemistry, Oligonucleotide, Reagent, Molecule, General Chemistry, Cross-conjugation, Combinatorial chemistry, DNA, Conjugate |
| الوصف: | The design of an orthogonal, thiol-reactive cross-conjugation reagent is presented that allows step-wise and site-selective combination of biomacromolecules such as proteins, peptides or oligonucleotides by simply adjusting the pH. Both reaction centers of this versatile bioconjugation reagent are located at opposite sites leaving sufficient space for combining even sterically demanding molecules. Our results are of high significance and general applicability for the preparation of defined peptide–protein, protein–protein or protein–DNA conjugates and pave the way to tailored biotherapeutics. |
| تدمد: | 2041-6539 2041-6520 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::b5e7e7a08f5ba1d3017d4268402f878dTest https://doi.org/10.1039/c3sc22015jTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........b5e7e7a08f5ba1d3017d4268402f878d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20416539 20416520 |
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