التفاصيل البيبلوغرافية
| العنوان: |
Protein inhibitor of activated signal transducer and activator of transcription (STAT)-1 (PIAS-1) regulates the IFN-γ response in macrophage cell lines |
| المؤلفون: |
Coccia, Eliana M.1 e.coccia@iss.it, Stellacci, Emilia2, Orsatti, Roberto2, Benedetti, Eleonora2, Giacomini, Elena1, Marziali, Giovanna2, Valdez, Benigno C.3, Battistini, Angela2 |
| المصدر: |
Cellular Signalling. Jun2002, Vol. 14 Issue 6, p537. 9p. |
| مصطلحات موضوعية: |
*MACROPHAGES, *CELL lines |
| مستخلص: |
Macrophage cell lines exhibit different responses to IFN-γ depending on their maturation stage. We investigated the mechanisms underlying the differential IFN-γ responsiveness in the less mature P388.D1 and in mature RAW264.7 cells. A reduction in the binding activity of the signal transducer and activator of transcription-1 (STAT-1) to different STAT binding elements (SBEs) was observed in P388.D1. This reduced binding activity was not due to an impaired STAT-1 activation. Studies on the expression of a negative regulator of cytokine signalling, protein-inhibiting activated STAT-1 (PIAS-1), showed that this protein was expressed constitutively at high levels in P388.D1. Forced expression of a PIAS-1 homologue, the Gu binding protein (GBP), inhibited the STAT-1-mediated gene activation in RAW264.7 cells, whereas a construct expressing the 5′ portion of GBP in the antisense orientation reverts the IFN-γ-resistant phenotype of P388.D1. Thus, our results indicate that PIAS-1 may account for the differential IFN-γ responsiveness in macrophage cell lines at different stages of maturation. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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