التفاصيل البيبلوغرافية
| العنوان: |
Gene Expression Profile in the Early Stage of Angiotensin II-induced Cardiac Remodeling: a Time Series Microarray Study in a Mouse Model. |
| المؤلفون: |
Dang, Meng-Qiu, Zhao, Xue-Chen, Lai, Song, Wang, Xia, Wang, Lei, Zhang, Yun-Long, Liu, Yang, Yu, Xiao-Hong, Liu, Ying, Li, Hui-Hua, Xia, Yun-Long |
| المصدر: |
Cellular Physiology & Biochemistry (Karger AG); Jan2015, Vol. 35 Issue 2, p467-476, 10p |
| مصطلحات موضوعية: |
GENE expression, ANGIOTENSIN II, VENTRICULAR remodeling, MICROARRAY technology, BLOOD pressure, ECHOCARDIOGRAPHY, LABORATORY mice |
| مستخلص: |
Background/Aims: Angiotensin II (Ang II) plays a critical role in the cardiac remodeling contributing to heart failure. However, the gene expression profiles induced by Ang II in the early stage of cardiac remodeling remain unknown. Methods: Wild-type male mice (C57BL/6 background, 10-weeek-old) were infused with Ang II (1500 ng/kg/min) for 7 days. Blood pressure was measured. Cardiac function and remodeling were examined by echocardiography, H&E and Masson staining. The time series microarrays were then conducted to detected gene expression profiles. Results: Microarray results identified that 1,489 genes were differentially expressed in the hearts at day 1, 3 and 7 of Ang II injection. These genes were further classified into 26 profiles by hierarchical cluster analysis. Of them, 4 profiles were significant (No. 19, 8, 21 and 22) and contained 904 genes. Gene Ontology showed that these genes mainly participate in metabolic process, oxidation-reduction process, extracellular matrix organization, apoptotic process, immune response, and others. Significant pathways included focal adhesion, ECM-receptor interaction, cytokine-cytokine receptor interaction, MAPK and insulin signaling pathways, which were known to play important roles in Ang II-induced cardiac remodeling. Moreover, gene co-expression networks analysis suggested that serine/cysteine peptidase inhibitor, member 1 (Serpine1, also known as PAI-1) localized in the core of the network. Conclusions: Our results indicate that many genes are mainly involved in metabolism, inflammation, cardiac fibrosis and hypertrophy. Serpine1 may play a central role in the development of Ang II-induced cardiac remodeling at the early stage. © 2015 S. Karger AG, Basel [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
