التفاصيل البيبلوغرافية
| العنوان: |
Resistin Enhances Monocyte Chemoattractant Protein-1 Production in Human Synovial Fibroblasts and Facilitates Monocyte Migration. |
| المؤلفون: |
Wei-Cheng Chen, Shih-Wei Wang, Chih-Yang Lin, Chun-Hao Tsai, Yi-Chin Fong, Ting-Yi Lin, Shun-Long Weng, Hsien-Da Huang, Kuang-Wen Liao, Chih-Hsin Tang |
| المصدر: |
Cellular Physiology & Biochemistry (Cell Physiol Biochem Press GmbH & Co. KG); 2019, Vol. 52 Issue 4, p408-420, 13p |
| مصطلحات موضوعية: |
RESISTIN, MONOCYTES, FIBROBLASTS, CELL migration, PHOSPHATIDYLINOSITOL 3-kinases, MICRORNA |
| مستخلص: |
Background/Aims: The adipocyte-secreting adipokine, resistin, may play a critical role in the modulation of inflammatory diseases. Migration and infiltration of mononuclear cells into inflammatory sites are critical events during the development of osteoarthritis (OA). Monocyte chemoattractant protein-1 (MCP-1), also known as chemokine ligand 2 (CCL2), plays a critical role in the regulation of monocyte migration and infiltration. In this study, we show how resistin promotes MCP-1 expression in OA synovial fibroblasts and monocyte migration. Methods: We used qPCR to detect MCP-1 and miRNA expression. THP-1 migration was investigated by Transwell assay. The Western blotting was used to examine the resistin-mediated signaling pathways. Results: Resistin activated the phosphatidylinositol-3-kinase (PI3K), Akt and mammalian target of rapamycin (mTOR) signaling pathways, while PI3K, Akt and mTOR inhibitors or small interfering RNAs diminished resistin-induced MCP-1 expression and monocyte migration. We also demonstrate that resistin stimulates MCP-1-mediated monocyte migration by suppressing microRNA (miR)-33a and miR-33b via the PI3K, Akt and mTOR signaling pathways. Conclusion: These results provide new insights into the mechanisms of resistin action that may have therapeutic implications for patients with OA. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
