Carnosine Protects Against Aβ42-induced Neurotoxicity in Differentiated Rat PC12 Cells
| العنوان: | Carnosine Protects Against Aβ42-induced Neurotoxicity in Differentiated Rat PC12 Cells |
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| المؤلفون: | Yanying Fan, Zhong Chen, Weiwei Hu, Yao Shen, Haibin Dai, Weiping Zhang, Qiuli Fu |
| المصدر: | Cellular and Molecular Neurobiology. 28:307-316 |
| بيانات النشر: | Springer Science and Business Media LLC, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Clobenpropit, medicine.drug_class, Neurotoxins, Glutamic Acid, Carnosine, Pharmacology, PC12 Cells, Receptors, N-Methyl-D-Aspartate, Phenoxypropanolamines, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Piperidines, medicine, Animals, Drug Interactions, Benzothiazoles, Neurons, Thioperamide, Amyloid beta-Peptides, Imidazoles, Thiourea, Neurotoxicity, Cell Differentiation, Cell Biology, General Medicine, Receptor antagonist, medicine.disease, Histidine decarboxylase, Peptide Fragments, Rats, Diphenhydramine, Histamine H2 Antagonists, nervous system, chemistry, Biochemistry, Histamine H1 Antagonists, NMDA receptor, Histamine, Histamine H3 Antagonists, medicine.drug |
| الوصف: | (1) The present study was designed to investigate whether histamine is involved in the protective effect of carnosine on Abeta42-induced impairment in differentiated PC12 cells. (2) PC12 cells were exposed to Abeta42 (5 muM) for 24 h after carnosine (5 mM) applied for 18 h. Histamine receptor antagonists (diphenhydramine, zolantidine, thioperamide, clobenpropit) or histidine decarboxylase inhibitor (alpha-fluoromethylhistidine) were added 15 min before carnosine. Cell viability, glutamate release or cell surface expression of NMDA receptor was examined. (3) Abeta42 caused a concentration-dependent reduction of viability in PC12 cells and pretreatment with carnosine ameliorated this impairment. This amelioration was reversed by the H(3) receptor antagonists thioperamide and clobenpropit, but not by either the H(1) receptor antagonist diphenhydramine or the H(2) receptor antagonist zolantidine. Further, alpha-fluoromethylhistidine, an irreversible inhibitor of histidine decarboxylase, also had no effect. In the presence of Abeta42, carnosine significantly decreased glutamate release and carnosine increased the surface expression of NMDA receptor. (4) These results indicate that the mechanism by which carnosine attenuates Abeta42-induced neurotoxicity is independent of the carnosine-histidine-histamine pathway, but may act through regulation of glutamate release and NMDA receptor trafficking. |
| تدمد: | 1573-6830 0272-4340 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bce5dc37300f3f11ac25acc878f79694Test https://doi.org/10.1007/s10571-007-9235-0Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....bce5dc37300f3f11ac25acc878f79694 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15736830 02724340 |
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