Impact of Pre-Analytical Factors on MSI Test Accuracy in Mucinous Colorectal Adenocarcinoma: A Multi-Assay Concordance Study
| العنوان: | Impact of Pre-Analytical Factors on MSI Test Accuracy in Mucinous Colorectal Adenocarcinoma: A Multi-Assay Concordance Study |
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| المؤلفون: | Caterina De Luca, Maria D'Armiento, Dario Bruzzese, Gianluca Russo, Mariangela Balestrieri, Antonio Bonfitto, Giancarlo Troncone, Pellegrino Cerino, Matteo Fassan, Maria Triassi, Umberto Malapelle, Filippo Pietrantonio, Claudia Covelli, Pasquale Pisapia, Fabiola Fiordelisi, Francesco Pepe, Paolo Graziano, Fotios Loupakis, Paola Parente |
| المساهمون: | Malapelle, U., Parente, P., Pepe, F., De Luca, C., Cerino, P., Covelli, C., Balestrieri, M., Russo, G., Bonfitto, A., Pisapia, P., Fiordelisi, F., D'Armiento, M., Bruzzese, D., Loupakis, F., Pietrantonio, F., Triassi, M., Fassan, M., Troncone, G., Graziano, P. |
| المصدر: | Cells, Vol 9, Iss 2019, p 2019 (2020) Cells Volume 9 Issue 9 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Oncology, Tissue Fixation, Colorectal cancer, microfluidic, Polymerase Chain Reaction, law.invention, 0302 clinical medicine, law, fully automated RT—PCR, Medicine, lcsh:QH301-705.5, Polymerase chain reaction, Pre analytical, DNA, Neoplasm, General Medicine, Middle Aged, Prognosis, Adenocarcinoma, Mucinous, Immunohistochemistry, Lynch syndrome, mCRC, 030220 oncology & carcinogenesis, IHC, predictive molecular pathology, Female, Microsatellite Instability, Colorectal Neoplasms, medicine.medical_specialty, Concordance, Article, Diagnosis, Differential, 03 medical and health sciences, Internal medicine, Humans, Aged, Retrospective Studies, Tissue Embedding, business.industry, Electrophoresis, Capillary, Microsatellite instability, medicine.disease, Colorectal Neoplasms, Hereditary Nonpolyposis, digestive system diseases, 030104 developmental biology, lcsh:Biology (General), Case-Control Studies, Neoplastic cell, business |
| الوصف: | Immunohistochemistry (IHC) and polymerase chain reaction (PCR) and fragment separation by capillary electrophoresis represent the current clinical laboratory standard for the evaluation of microsatellite instability (MSI) status. The importance of reporting MSI status in colorectal cancer is based on its potential for guiding treatment and as a prognostic indicator. It is also used to identify patients for Lynch syndrome testing. Our aim was to evaluate pre-analytical factors, such as age of formalin-fixed and paraffin-embedded (FFPE) block, neoplastic cell percentage, mucinous component, and DNA integrity, that may influence the accuracy of MSI testing and assess the concordance between three different MSI evaluation approaches. We selected the mucinous colorectal cancer (CRC) histotype for this study as it may possibly represent an intrinsic diagnostic issue due to its low tumor cellularity. Seventy-five cases of mucinous CRC and corresponding normal colon tissue samples were retrospectively selected. MMR proteins were evaluated by IHC. After DNA quality and quantity evaluation, the Idylla&trade and TapeStation 4200 platforms were adopted for the evaluation of MSI status. Seventy-three (97.3%) cases were successfully analyzed by the three methodologies. Overall, the Idylla&trade platform showed a concordance rate with IHC of 98.0% for microsatellite stable (MSS)/proficient MMR (pMMR) cases and 81.8% for MSI/deficient MMR (dMMR) cases. The TapeStation 4200 system showed a concordance rate with IHC of 96.0% for MSS/pMMR cases and 45.4% for MSI/dMMR cases. The concordance rates of the TapeStation 4200 system with respect to the Idylla&trade platform were 98.1% for MSS profile and 57.8% for MSI profile. Discordant cases were analyzed using the Titano MSI kit. Considering pre-analytical factors, no significant variation in concordance rate among IHC analyses and molecular systems was observed by considering the presence of an acellular mucus cut-off > 50% of the tumor area, FFPE year preparation, and DNA concentration. Conversely, the Idylla&trade platform showed a significant variation in concordance rate with the IHC approach by considering a neoplastic cell percentage > 50% (p-value = 0.002), and the TapeStation 4200 system showed a significant variation in concordance rate with the IHC approach by considering a DNA integrity number (DIN) &ge 4 as cut-off (p-value = 0.009). Our data pinpoint a central role of the pre-analytical phase in the diagnostic outcome of MSI testing in CRC. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4409 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69d62a61c32796b9d18a796ec72c503fTest https://www.mdpi.com/2073-4409/9/9/2019Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....69d62a61c32796b9d18a796ec72c503f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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