Harnessing the Power of Induced Pluripotent Stem Cells and Gene Editing Technology: Therapeutic Implications in Hematological Malignancies
| العنوان: | Harnessing the Power of Induced Pluripotent Stem Cells and Gene Editing Technology: Therapeutic Implications in Hematological Malignancies |
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| المؤلفون: | Sonali P. Barwe, Raju Pillai, Ishnoor Sidhu, Anilkumar Gopalakrishnapillai |
| المصدر: | Cells, Vol 10, Iss 2698, p 2698 (2021) Cells |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | QH301-705.5, Induced Pluripotent Stem Cells, Computational biology, Disease, Review, Biology, Hematopoietic lineage, Somatic evolution in cancer, Genome editing, Disease severity, medicine, Animals, Humans, hematologic, Biology (General), Induced pluripotent stem cell, Clinical Trials as Topic, iPSC, Mechanism (biology), gene editing, Cancer, General Medicine, medicine.disease, Hematopoiesis, Disease Models, Animal, Hematologic Neoplasms |
| الوصف: | In vitro modeling of hematological malignancies not only provides insights into the influence of genetic aberrations on cellular and molecular mechanisms involved in disease progression but also aids development and evaluation of therapeutic agents. Owing to their self-renewal and differentiation capacity, induced pluripotent stem cells (iPSCs) have emerged as a potential source of short in supply disease-specific human cells of the hematopoietic lineage. Patient-derived iPSCs can recapitulate the disease severity and spectrum of prognosis dictated by the genetic variation among patients and can be used for drug screening and studying clonal evolution. However, this approach lacks the ability to model the early phases of the disease leading to cancer. The advent of genetic editing technology has promoted the generation of precise isogenic iPSC disease models to address questions regarding the underlying genetic mechanism of disease initiation and progression. In this review, we discuss the use of iPSC disease modeling in hematological diseases, where there is lack of patient sample availability and/or difficulty of engraftment to generate animal models. Furthermore, we describe the power of combining iPSC and precise gene editing to elucidate the underlying mechanism of initiation and progression of various hematological malignancies. Finally, we discuss the power of iPSC disease modeling in developing and testing novel therapies in a high throughput setting. |
| اللغة: | English |
| تدمد: | 2073-4409 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ea8e6ad8fd4974b84e3874df63dd7e4Test https://www.mdpi.com/2073-4409/10/10/2698Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2ea8e6ad8fd4974b84e3874df63dd7e4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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