Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly
| العنوان: | Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly |
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| المؤلفون: | Tara R. Richman, Stanka Matic, Ilian Atanassov, Dusanka Milenkovic, Judith A. Ermer, Oliver Rackham, Aleksandra Filipovska, James B. Stewart, Jakob D. Busch, Irina M. Kuznetsova, Anne-Marie J. Shearwood, Nils-Göran Larsson, Arnaud Mourier, Stefan J. Siira |
| المصدر: | Cell Reports Cell Rep Cell Reports, Vol 16, Iss 7, Pp 1874-1890 (2016) |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Ribosomal Proteins, PPR domains, Mitochondrial RNA processing, Transcription, Genetic, TRNA processing, Ribosome biogenesis, Biology, Cell Fractionation, General Biochemistry, Genetics and Molecular Biology, Mitochondria, Heart, Ribonuclease P, Mitochondrial Proteins, Mitochondrial Ribosomes, 03 medical and health sciences, Mice, 0302 clinical medicine, RNA, Transfer, RNA, Ribosomal, 16S, Animals, RNA Processing, Post-Transcriptional, Muscle, Skeletal, lcsh:QH301-705.5, RNA metabolism, Genetics, Mitochondrial ribosome assembly, Mice, Knockout, Organelle Biogenesis, Myocardium, RNA, Non-coding RNA, Cell biology, mitochondria, Mice, Inbred C57BL, 030104 developmental biology, lcsh:Biology (General), RNA editing, Protein Biosynthesis, Transfer RNA, RNA-seq, Cardiomyopathies, Transcriptome, 030217 neurology & neurosurgery |
| الوصف: | Summary The regulation of mitochondrial RNA processing and its importance for ribosome biogenesis and energy metabolism are not clear. We generated conditional knockout mice of the endoribonuclease component of the RNase P complex, MRPP3, and report that it is essential for life and that heart and skeletal-muscle-specific knockout leads to severe cardiomyopathy, indicating that its activity is non-redundant. Transcriptome-wide parallel analyses of RNA ends (PARE) and RNA-seq enabled us to identify that in vivo 5′ tRNA cleavage precedes 3′ tRNA processing, and this is required for the correct biogenesis of the mitochondrial ribosomal subunits. We identify that mitoribosomal biogenesis proceeds co-transcriptionally because large mitoribosomal proteins can form a subcomplex on an unprocessed RNA containing the 16S rRNA. Taken together, our data show that RNA processing links transcription to translation via assembly of the mitoribosome. |
| تدمد: | 2211-1247 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c1914052604de7b1cbe554b15867e94Test https://pubmed.ncbi.nlm.nih.gov/27498866Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8c1914052604de7b1cbe554b15867e94 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22111247 |
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